A marine-derived Streptomyces sp MS449 produces high yield of actinomycin X2 and actinomycin D with potent anti-tuberculosis activity
A marine-derived Streptomyces sp MS449 produces high yield of actinomycin X2 and actinomycin D with potent anti-tuberculosis activity
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DOI：
10.1007/s00253-012-4079-z
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发表时间：
2012-08-01
影响因子：
5
通讯作者：
Zhang, Lixin
中科院分区：
文献类型：
--
作者：
Chen, Caixia;Song, Fuhang;Zhang, Lixin
在从海洋天然产物库中筛选抗牛分枝杆菌（MycobacteriumbovisbacillusCalmette-Gu，rin，BCG）和抗结核分枝杆菌（Mycobacteriumtuberculosis，MTB）H37 Rv的过程中，筛选到一株新分离的放线菌MS 449，命名为MS 449。从南海沉积物中分离到的菌株MS 449能产生大量的放线菌素X-2和放线菌素D，对BCG和MTB H37 Rv有较强的抑制作用。通过核磁共振和质谱分析确定了放线菌素的结构。菌株MS 449的形态和表型特征，基因型数据和系统发育分析的基础上进行了分类特征。测定了该菌株的16 S rRNA基因序列，并对数据库进行检索，结果表明该菌株与阿维链霉菌的模式菌株关系密切（16 S rRNA基因相似性为99.7%）。S.以前没有报道阿维菌素产生放线菌素。海洋来源的链霉菌MS 449菌株产生的放线菌素X-2（1.92 mg/ml）和放线菌素D（1.77 mg/ml）的量显著高于先前报道的放线菌素产生菌株。因此，MS 449具有作为放线菌素X-2和放线菌素D工业生产新菌株的巨大潜力。
In the course of our screening program for anti-Mycobacterium bovis bacillus Calmette-Gu,rin (BCG) and anti-Mycobacterium tuberculosis H37Rv (MTB H37Rv) agents from our marine natural product library, a newly isolated actinomycete strain, designated as MS449, was picked out for further investigation. The strain MS449, isolated from a sediment sample collected from South China Sea, produced actinomycin X-2 and actinomycin D in substantial quantities, which showed strong inhibition of BCG and MTB H37Rv. The structures of actinomycins were elucidated by nuclear magnetic resonance and mass spectrometric analysis. The strain MS449 was taxonomically characterized on the basis of morphological and phenotypic characteristics, genotypic data, and phylogenetic analysis. The 16S rRNA gene sequence of the strain was determined and a database search indicated that the strain was closely associated with the type strain of Streptomyces avermitilis (99.7 % 16S rRNA gene similarity). S. avermitilis has not been previously reported to produce actinomycins. The marine-derived strain of Streptomyces sp. MS449 produced notably higher quantities of actinomycin X-2 (1.92 mg/ml) and actinomycin D (1.77 mg/ml) than previously reported actinomycins producing strains. Thus, MS449 was considered of great potential as a new industrial producing strain of actinomycin X-2 and actinomycin D.