REGULATORY GENE-EXPRESSION BOUNDARIES DEMARCATE SITES OF NEURONAL DIFFERENTIATION IN THE EMBRYONIC ZEBRAFISH FOREBRAIN
REGULATORY GENE-EXPRESSION BOUNDARIES DEMARCATE SITES OF NEURONAL DIFFERENTIATION IN THE EMBRYONIC ZEBRAFISH FOREBRAIN
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DOI：
10.1016/0896-6273(94)90044-2
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发表时间：
1994-11-01
期刊：
影响因子：
16.2
通讯作者：
WILSON, SW
中科院分区：
文献类型：
--
作者：
MACDONALD, R;XU, QL;WILSON, SW
在斑马鱼前脑发育过程中，一个简单的轴突通路支架是由少数神经元率先形成的。我们发现eph、forkhead、pax和wnt基因家族成员的表达域边界与这些神经元分化和延伸轴突的位置相关。对遗传或实验改变的前脑的分析表明，如果保持一个边界，则存在有关边界的适当神经分化。相反，在没有边界的情况下，神经模式也会随之中断。我们还发现，间脑背侧的一条带细胞与腹侧中线细胞具有相同的特征。在腹侧中枢神经系统被破坏的突变鱼类中，这条细胞不能发育，这表明它的发育可能受到与腹侧中线相同的诱导通路的调节。
During development of the zebrafish forebrain, a simple scaffold of axon pathways is pioneered by a small number of neurons. We show that boundaries of expression domains of members of the eph, forkhead, pax, and wnt gene families correlate with the positions at which these neurons differentiate and extend axons. Analysis of genetically or experimentally altered forebrains indicates that if a boundary is maintained, there is appropriate neural differentiation with respect to the boundary. Conversely, in the absence of a boundary, there is concomitant disruption of neural patterning. We also show that a strip of cells within the dorsal diencephalon shares features with ventral midline cells. This strip of cells fails to develop in mutant fish in which specification of the ventral CNS is disrupted, suggesting that its development may be regulated by the same inductive pathways that pattern the ventral midline.