In situ high-resolution atomic force microscope imaging of biological surfaces
In situ high-resolution atomic force microscope imaging of biological surfaces
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DOI：
10.1116/1.580370
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发表时间：
1996-05-01
影响因子：
2.9
通讯作者：
Henderson, GS
中科院分区：
文献类型：
--
作者：
Sokolov, IY;Firtel, M;Henderson, GS
生物表面的原位高分辨率原子力显微镜成像在具有相对刚性表面的细胞上进行（例如，细菌）。瑞士乳杆菌（一种杆状细菌）的表面进行了研究之前和之后暴露于氯化锂，变性剂。在不同的力负荷和不同的扫描方向下，图像细节都是稳定的。从倾斜晶格结构的图像（即，S层L. helveticus），估计图像的横向分辨率高达2nm。该分辨率可以通过假设在尖端的端部附近存在具有类似于10 nm的半径的曲率的顶点来解释。这种几何形状的建模表明，这样的尖端配置是特别适合于原位高分辨率成像的刚性壳（膜）覆盖的相对柔软的对象。(C)1996年美国真空学会。
In situ high-resolution atomic force microscope imaging of biological surfaces was performed on cells with relatively rigid surfaces (e.g., bacteria). The surface of Lactobacillus helveticus (a rod-shaped bacterium) was investigated before and after exposure to LiCl, a denaturant. Image details were stable both at variant force loads and under different scan directions. From images of the oblique lattice structure (i.e., S layer of L. helveticus), it was estimated that the lateral resolution of the images was up to 2 nm. This resolution can be explained by assuming that there is an apex with a curvature of radius of similar to 10 nm near the end of the tip. Modelling of this geometry indicates that such a tip configuration is particularly suitable for in situ high-resolution imaging of relatively soft objects covered by a rigid shell (membrane). (C) 1996 American Vacuum Society.