ATONAL IS A PRONEURAL GENE THAT DIRECTS CHORDOTONAL ORGAN FORMATION IN THE DROSOPHILA PERIPHERAL NERVOUS-SYSTEM
ATONAL IS A PRONEURAL GENE THAT DIRECTS CHORDOTONAL ORGAN FORMATION IN THE DROSOPHILA PERIPHERAL NERVOUS-SYSTEM
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DOI：
10.1016/0092-8674(93)90358-w
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发表时间：
1993-07-02
期刊：
影响因子：
64.5
通讯作者：
JAN, YN
中科院分区：
文献类型：
--
作者：
JARMAN, AP;GRAU, Y;JAN, YN
在果蝇的周围神经系统中，无毛-鳞状复合体(ASC)的原神经基因是形成外感觉器官前体所必需的，而不是脊索性器官的前体。我们报告分离到一个基因无音(ATO)，有证据表明它是一个与脉络膜器官形成有关的原神经性基因。该基因在神经丛和感觉器官前体中表达，这些前体产生胚胎和成体的脉络膜器官，而不是外部感觉器官。在携带移除ATO的染色体缺陷的胚胎中，脊索器官被消除。与ASC产物一样，ATO蛋白含有一个碱性-螺旋-环-螺旋区域，并与无子蛋白异源二聚体结合到E盒上。此外，ATO的异位表达促进了额外感觉器官的形成。尽管具有相似的神经特性，但我们发现ASC基因的异位表达仅促进外部感觉器官的形成，而ATO优先促进脉络膜器官的形成。因此，原神经基因是神经元同一性的主要决定因素。
In the Drosophila peripheral nervous system, proneural genes of the achaete-scute complex (ASC) are required for formation of the precursors of external sense organs but not of chordotonal organs. We report the isolation of a gene, atonal (ato), with evidence that it is a proneural gene for the formation of chordotonal organs. This gene is expressed in the proneural clusters and sense organ precursors that give rise to the embryonic and adult chordotonal, but not external sense, organs. Chordotonal organs are eliminated in embryos carrying chromosomal deficiencies that remove ato. Like the ASC products, ato protein contains a basic-helix-loop-helix region and heterodimerizes with daughterless protein to bind to E boxes. Moreover, ectopic expression of ato promotes the formation of extra sense organs. Despite similar proneural properties, we find that ectopic expression of the ASC genes promotes external sense organ formation exclusively, whereas ato promotes chordotonal organ formation preferentially. Thus, proneural genes are major determinants of neuronal identity.