Silver nanoparticle-coated self-expandable metallic stent suppresses tissue hyperplasia in a rat esophageal model
Silver nanoparticle-coated self-expandable metallic stent suppresses tissue hyperplasia in a rat esophageal model
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银纳米颗粒涂层自膨胀金属支架抑制大鼠食管模型中的组织增生
DOI：
10.1007/s00464-020-08238-4
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发表时间：
2022
影响因子：
3.1
通讯作者：
Li Xiao
中科院分区：
文献类型：
--
作者：
Zhao He;Fu Yan;Tsauo Jiaywei;Zhang Xiaowu;Zhao Yanqing;Gong Tao;Li Jingui;Li Xiao
BackgroundTo evaluate a efficacy of a银纳米粒子（AgNP）涂层的自膨胀金属支架（SEMS）用于抑制组织增生在大鼠食管model.Methods24只雄性Sprague-Dawley大鼠随机分为四组。A组动物接受无涂层SEMS放置，而B、C和D组动物分别接受6、12和24 mg/mL AgNP涂层SEMS放置。所有动物安乐死4周后SEMS放置，和一个大体检查和组织学analysiswere performed.ResultsAll大鼠取得了技术上的成功，并存活到研究结束。大体检查显示A组5只大鼠和B组2只大鼠中重度组织增生。相比之下，C组和D组中没有动物具有中度或重度组织增生。大体检查未发现并发症。C组和D组肉芽组织面积百分比、上皮层数、黏膜下纤维化厚度、结缔组织面积百分比、炎细胞浸润程度、胶原沉积程度、Ki 67、TUNEL和α-SMA阳性沉积程度均显著低于A组（p < 0.05）。B组仅肉芽组织面积百分比、上皮层数、粘膜下纤维化厚度和结缔组织面积百分比显著低于A组（均P < 0.05）。D组和C组之间的组织学参数无显著差异（均P> 0.05）。结论AgNP包被的SEMSs抑制大鼠食管模型中的组织增生。
BackgroundTo evaluate the efficacy of a silver nanoparticle (AgNP)-coated self-expandable metallic stent (SEMS) for suppressing tissue hyperplasia in a rat esophageal model.MethodsTwenty-four male Sprague-Dawley rats were randomly assigned to four groups. Animals in group A underwent uncoated SEMS placement, whereas animals in groups B, C, and D underwent 6, 12, and 24 mg/mL AgNP-coated SEMS placement, respectively. All animals were euthanized 4 weeks after SEMS placement, and a gross examination and histological analyses were performed.ResultsAll rats achieved technical success and survived until the end of the study. The gross examination showed moderate to severe tissue hyperplasia in 5 rats in group A and 2 rats in group B. In contrast, no animals in groups C and D had moderate or severe tissue hyperplasia. The gross examination revealed no complications. The percentage of granulation tissue area, number of epithelial layers, thickness of submucosal fibrosis, percentage of connective tissue area, inflammatory cell infiltration grade, degree of collagen deposition, and degrees of Ki67, TUNEL, and α-SMA-positive deposition were significantly lower in groups C and D than in group A (allp< 0.05). However, only the percentage of granulation tissue area, number of epithelial layers, thickness of submucosal fibrosis, and percentage of connective tissue area were significantly lower in group B than in group A (allp< 0.05). No histological parameters were significantly different between group D and group C (allp> 0.05).ConclusionAgNP-coated SEMSs suppressed tissue hyperplasia in a rat esophageal model.