Characterizing local collagen fiber re-alignment and crimp behavior throughout mechanical testing in a mature mouse supraspinatus tendon model
Characterizing local collagen fiber re-alignment and crimp behavior throughout mechanical testing in a mature mouse supraspinatus tendon model
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DOI：
10.1016/j.jbiomech.2012.06.006
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发表时间：
2012-08-09
影响因子：
2.4
通讯作者：
Soslowsky, Louis J.
中科院分区：
文献类型：
--
作者：
Miller, Kristin S.;Connizzo, Brianne K.;Soslowsky, Louis J.
背景：胶原纤维重新排列和解卷曲是肌腱结构对载荷反应的两种假设机制。最近的研究已经检查了出生后发育小鼠冈上肌腱模型（SST）中响应于机械测试的结构变化，然而，成熟小鼠中的这些变化尚未被表征。本研究的目的是表征胶原纤维重新排列和卷曲的行为在整个机械测试在一个成熟的小鼠SST.Method的方法：一个拉伸力学测试设置集成了偏振光系统用于对齐和力学分析。使用传统的拉伸设置和快速冷冻方法，在指定的加载方案后立即定量局部胶原纤维卷曲频率。预处理周期数对胶原纤维重新排列，卷曲频率和机械性能在midsubstance和插入部位locations.Results的影响：减少胶原纤维卷曲频率被确定在脚趾区域的机械测试在这两个位置。插入部位在整个试验过程中重新对齐，而中间物质在预处理和试验线性区域期间重新对齐。插入部位表现出更混乱的胶原纤维分布，较低的机械性能和较高的横截面积相比，midsubstance location.Conclusions：本地胶原纤维重新排列，卷曲行为和机械性能的特点是在一个成熟的小鼠SST模型。插入位点和中间物质对机械载荷的响应不同，并且具有不同的结构响应机制。此外，结果支持胶原纤维卷曲是一种生理现象，可以解释机械测试脚趾区域。(C)2012爱思唯尔有限公司保留所有权利。
Background: Collagen fiber re-alignment and uncrimping are two postulated mechanisms of tendon structural response to load. Recent studies have examined structural changes in response to mechanical testing in a postnatal development mouse supraspinatus tendon model (SST), however, those changes in the mature mouse have not been characterized. The objective of this study was to characterize collagen fiber re-alignment and crimp behavior throughout mechanical testing in a mature mouse SST.Method of approach: A tensile mechanical testing set-up integrated with a polarized light system was utilized for alignment and mechanical analysis. Local collagen fiber crimp frequency was quantified immediately following the designated loading protocol using a traditional tensile set up and a flash-freezing method. The effect of number of preconditioning cycles on collagen fiber re-alignment, crimp frequency and mechanical properties in midsubstance and insertion site locations were examined.Results: Decreases in collagen fiber crimp frequency were identified at the toe-region of the mechanical test at both locations. The insertion site re-aligned throughout the entire test, while the midsubstance re-aligned during preconditioning and the test's linear-region. The insertion site demonstrated a more disorganized collagen fiber distribution, lower mechanical properties and a higher cross-sectional area compared to the midsubstance location.Conclusions: Local collagen fiber re-alignment, crimp behavior and mechanical properties were characterized in a mature mouse SST model. The insertion site and midsubstance respond differently to mechanical load and have different mechanisms of structural response. Additionally, results support that collagen fiber crimp is a physiologic phenomenon that may explain the mechanical test toe-region. (C) 2012 Elsevier Ltd. All rights reserved.