Adult hippocampal neurogenesis and voluntary running activity: Circadian and dose-dependent effects
Adult hippocampal neurogenesis and voluntary running activity: Circadian and dose-dependent effects
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DOI：
10.1002/jnr.20039
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发表时间：
2004-04-15
影响因子：
4.2
通讯作者：
Kempermann, G
中科院分区：
文献类型：
--
作者：
Holmes, MM;Galea, LAM;Kempermann, G
跑步活动增加成年小鼠齿状回的细胞增殖和神经发生。本实验旨在研究活动对成年神经发生的影响是否依赖于一天中的时间（昼夜节律阶段）和活动量。小鼠在一天中的三个时间之一接受对转轮的限制性访问（0、1或3小时）：光相的中间（即，当小鼠通常不活动时），暗开始（即，当小鼠开始它们的夜间活动时），和黑暗期的中间（即，当小鼠处于其活跃期的中间时）。细胞增殖和净神经发生后，掺入胸苷类似物溴脱氧尿苷（BrdU）和免疫组化检测BrdU和神经元标记物进行了评估。运行活动显着增加细胞增殖，细胞存活，和新的神经元的总数只有在动物3小时的车轮访问期间的黑暗时期的中间。虽然在所有时间点的活动与神经发生增加呈正相关，但在黑暗期开始时或光照期中期，在车轮进入的动物中，这种影响在统计学上并不显著。这些数据表明，运动对细胞增殖和神经发生的影响是由昼夜节律和每日运动量调节的，从而为生理和行为因素之间的复杂关系提供了新的见解，这些因素可以介导成人神经可塑性。(C)2004威利-利斯公司
Running activity increases cell proliferation and neurogenesis in the dentate gyrus of adult mice. The present experiment was designed to investigate whether the effect of activity on adult neurogenesis is dependent on the time of day (circadian phase) and the amount of activity. Mice received restricted access to a running wheel (0, 1, or 3 hr) at one of three times of day: the middle of the light phase (i.e., when mice are normally inactive), dark onset (i.e., when mice begin their nocturnal activity), and the middle of the dark period (i.e., when mice are in the middle of their active period). Cell proliferation and net neurogenesis were assessed after incorporation of the thymidine analog bromocdoxyuridine (BrdU) and immunohistochemical detection of BrdU and neuronal markers. Running activity significantly increased cell proliferation, cell survival, and total number of new neurons only in animals with 3 hr of wheel access during the middle of the dark period. Although activity was positively correlated with increased neurogenesis at all time points, the effects were not statistically significant in animals with wheel access at the beginning of the dark period or during the middle of the light period. These data suggest that the influence of exercise on cell proliferation and neurogenesis is modulated by both circadian phase and the amount of daily exercise, thus providing new insight into the complex relationship between physiological and behavioral factors that can mediate adult neuroplasticity. (C) 2004 Wiley-Liss, Inc.