Cellular taxonomy and spatial organization of the murine ventral posterior hypothalamus.
Cellular taxonomy and spatial organization of the murine ventral posterior hypothalamus.
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DOI：
10.7554/elife.58901
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发表时间：
2020-10-29
期刊：
影响因子：
7.7
通讯作者：
Jackson AC
中科院分区：
文献类型：
--
作者：
Mickelsen LE;Flynn WF;Springer K;Wilson L;Beltrami EJ;Bolisetty M;Robson P;Jackson AC
腹后下丘脑（VPH）是一个解剖学上复杂的大脑区域，涉及唤醒，生殖，能量平衡和记忆处理。然而，VPH内神经元细胞类型的多样性知之甚少，阻碍了解构不同VPH回路在生理和行为中的作用。为了解决这个问题，我们采用了基于液滴的单细胞RNA测序（scRNA-seq）方法来系统地分类小鼠VPH中分子上不同的细胞群。对> 16，000个单细胞的分析揭示了20个神经元和18个非神经元细胞群，其由一套区分性标记物定义。我们通过荧光原位杂交（FISH）验证了所选神经元群体中差异表达的基因。专注于乳头体（MB），我们发现转录不同的集群，表现出MB细分和地形投影到丘脑内的神经解剖学parcellation。这种VPH细胞类型的单细胞转录组图谱为询问VPH电路的不同功能的电路水平机制提供了资源。
The ventral posterior hypothalamus (VPH) is an anatomically complex brain region implicated in arousal, reproduction, energy balance, and memory processing. However, neuronal cell type diversity within the VPH is poorly understood, an impediment to deconstructing the roles of distinct VPH circuits in physiology and behavior. To address this question, we employed a droplet-based single-cell RNA sequencing (scRNA-seq) approach to systematically classify molecularly distinct cell populations in the mouse VPH. Analysis of >16,000 single cells revealed 20 neuronal and 18 non-neuronal cell populations, defined by suites of discriminatory markers. We validated differentially expressed genes in selected neuronal populations through fluorescence in situ hybridization (FISH). Focusing on the mammillary bodies (MB), we discovered transcriptionally-distinct clusters that exhibit neuroanatomical parcellation within MB subdivisions and topographic projections to the thalamus. This single-cell transcriptomic atlas of VPH cell types provides a resource for interrogating the circuit-level mechanisms underlying the diverse functions of VPH circuits.