The Arabidopsis thaliana AT PRP39-1 gene, encoding a tetratricopeptide repeat protein with similarity to the yeast pre-mRNA processing protein PRP39, affects flowering time
The Arabidopsis thaliana AT PRP39-1 gene, encoding a tetratricopeptide repeat protein with similarity to the yeast pre-mRNA processing protein PRP39, affects flowering time
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DOI：
10.1007/s00299-007-0336-5
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发表时间：
2007-03
影响因子：
6.2
通讯作者：
Cunxi Wang;Qing Tian;Zhenglin Hou;M. Mucha;M. Aukerman;O. Olsen
中科院分区：
文献类型：
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作者：
Cunxi Wang;Qing Tian;Zhenglin Hou;M. Mucha;M. Aukerman;O. Olsen
植物的开花受光周期、春化、赤霉素和自主通路等四种通路的综合调控。众所周知，自主通路中的RNA加工可以调节拟南芥的开花时间。在这里，我们发现了一个新的拟南芥基因，命名为at PRP39-1，影响开花时间。基于对纯合子prp39-1植株的观察，我们初步认为，at prp39-1可能是该自主通路的一个新组成部分。与先前对自主通路基因的研究一致，敲除at prp39 -1在拟南芥中表现出稳态水平的ofFLC上调，同时在成年组织中下调offtandsoc1转录本水平。AT prp39 -1编码一个与酵母mRNA加工蛋白Prp39p相似的四肽重复蛋白，这表明这些四肽重复蛋白参与RNA加工在酵母、人类和植物中是保守的。结构建模表明AT PRP39-1具有两个适合靶蛋白结合的TPR超螺旋结构域。我们讨论了prp39 -1如何在自主通路的背景下控制开花。
Flowering is regulated by a network integrated from four major pathways, including the photoperiod, vernalization, gibberellin, and autonomous pathways. RNA processing within the autonomous pathway is well known to regulateArabidopsis thalianaflowering time. Here we identify a novelArabidopsisgene, designatedAT PRP39-1, that affects flowering time. Based on observations that homozygousat prp39-1plants are late flowering under both long and short days and responsive to GA and vernalization treatment, we tentatively conclude thatAT PRP39-1may represent a new component of the autonomous pathway. Consistent with previous studies on genes of the autonomous pathway, knockout ofAT PRP39-1inArabidopsisdisplays an upregulation of the steady state level ofFLC, and simultaneous downregulation ofFTandSOC1transcript levels in adult tissues.AT PRP39-1encodes a tetratricopeptide repeat protein with a similarity to a yeast mRNA processing protein Prp39p, suggesting that the involvement of these tetratricopeptide repeat proteins in RNA processing is conserved among yeast, human, and plants. Structure modeling suggests that AT PRP39-1 has two TPR superhelical domains suitable for target protein binding. We discuss howAT PRP39-1may function in the control of flowering in the context of the autonomous pathway.