Use of FTIR spectroscopy and PCA-LDC analysis to identify cancerous lesions within the human colon
Use of FTIR spectroscopy and PCA-LDC analysis to identify cancerous lesions within the human colon
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DOI：
10.1016/j.jpba.2016.11.047
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发表时间：
2017-02-05
影响因子：
3.4
通讯作者：
Cebulski, J.
中科院分区：
文献类型：
--
作者：
Kaznowska, E.;Depciuch, J.;Cebulski, J.
结直肠癌占所有癌症发病率的33%，因此对结直肠癌诊断和化疗监测的新方法的研究正在如火如荼地进行。人们正在寻找诊断仪器，这种仪器能够基于对组织材料的分析来检测单个恶性细胞，这些组织材料可能在诊断管理的进一步阶段重复使用。最常见的组织标本处理方法是石蜡包埋。然而，石蜡在光谱测量中可能会产生背景噪声，波数范围在900厘米(-1)到3500厘米(-1)之间。然而，Depciuch等人的研究。(2016)证明，适当的标本处理和石蜡包埋技术以及严格的测量方法可以消除石蜡振动。因此，只要保持高标准的标本处理，光谱测量可能成为一种可靠和精确的方法，用于结直肠癌患者的诊断和治疗监测。化疗是结直肠癌的主要内科治疗方法。不幸的是，缺乏能够监测其疗效的工具导致了受影响患者经常出现的部分应答或无应答。因此，也在寻求能够监测治疗效果的诊断仪器，以便能够及早改变化疗方案，从而增加治愈的机会。本文旨在比较几种类型的结肠组织：正常结肠、癌变结肠、化疗后结肠和正常结肠癌手术切缘的FTIR(傅立叶变换红外光谱)结果。获得的FTIR光谱以及主成分分析-线性判别分析(PCA-LDC)以及初级酰胺区域的带宽分析表明，健康组织的光谱与癌症组织(化疗前或化疗后)之间存在一些差异。除了证实FTIR光谱是分析样本成分的良好信息来源外，这一事实还支持将其作为一种工具，以促进了解各种情况的病理生理学和监测癌症患者的化疗效果。(C)2016爱思唯尔B.V.保留所有权利。
Colorectal cancer constitutes 33% of all cancer morbidity, so the research of the new methods for colorectal cancer diagnosis and chemotherapy monitoring is gaining its momentum. Diagnostic instruments are being sought, which enable the detection of single malignant cells based on the analysis of tissue material potentially reusable at further stages of diagnostic management. The most common approach to tissue specimen processing is paraffin-embedding. Yet, paraffin may cause background noise in spectroscopic measurements with the wavenumber ranging between 900 cm(-1) and 3500 cm(-1). However, the study by Depciuch et al. (2016) proved that appropriate specimen processing and paraffin-embedding technique as well as a strict measurement methodology may eliminate paraffin vibrations. As a result, spectroscopic measurements may become a reliable and precise method for the diagnosis and treatment monitoring in patients with colorectal cancer as long as the high standards of specimen processing are maintained. Chemotherapy is the main medical treatment in colorectal cancer. Unfortunately, the absence of tools which enable monitoring its efficacy leads to the partial response or non-response frequently seen in affected patients. Hence, diagnostic instruments are also being sought capable of monitoring treatment efficacy so as to enable early changes of chemotherapy regimen thus increasing the chance of cure. The paper aims at comparing the results of FTIR (Fourier Transform Infrared) spectroscopy in several types of colon tissue: healthy colon, cancerous colon, post-chemotherapy colon and healthy surgical margin of colon cancer sample. The obtained FTIR spectra along with the Principal Component Analysis-Linear Discriminant Analysis (PCA-LDC) as well as bandwidth analysis of the primary amide region revealed some differences between the spectra of healthy tissues as compared to cancerous tissues (pre- or post chemotherapy). Apart from confirming that FTIR spectroscopy is a good source of information on the composition of analysed samples, this fact supports its application as a tool to facilitate understanding the pathophysiology of various conditions and to monitor efficacy of chemotherapy in cancer patients. (C) 2016 Elsevier B.V. All rights reserved.