A novel crustin from Marsupenaeus japonicus promotes hemocyte phagocytosis
A novel crustin from Marsupenaeus japonicus promotes hemocyte phagocytosis
复制标题
日本袋对虾的一种新型外壳蛋白可促进血细胞吞噬作用。
DOI：
10.1016/j.dci.2014.11.021
复制
发表时间：
2015-04-01
影响因子：
2.9
通讯作者：
Wang, Jin-Xing
中科院分区：
文献类型：
--
作者：
Liu, Ning;Lan, Jiang-Feng;Wang, Jin-Xing
甲壳素是阳离子富含半胱氨酸的抗微生物肽（AMPS），其在N-末端含有多个结构域（富含甘氨酸、富含半胱氨酸或富含脯氨酸），在C-末端含有乳清酸性蛋白（WAP）结构域。甲壳具有多种功能，包括蛋白酶抑制和抗菌活性。甲壳的其他功能需要澄清。在这项研究中，一个新的crustin与半胱氨酸丰富的地区，和一个单一的WAP域，属于I型甲壳，被确定为MjCru I-1的Marsupenaeus palaeus。MjCru I-1在多种组织中表达。MjCru I-1在细菌感染的对虾血细胞中表达上调。MjCru I-1可以通过与细菌的细胞壁分子如脂多糖（LPS）、肽聚糖（PGN）和脂磷壁酸（LTA）结合而与细菌结合。合成的MjCru I-1的WAP结构域具有抗菌和凝集活性，而非合成的Cys富结构域。扫描电镜分析显示，与对照样品相比，经sMjCru I-1处理的细菌细胞出现破裂和破裂。敲低MjCru I-1基因可降低细菌清除率，注射MjCru I-1可显著提高感染鳗弧菌和金黄色葡萄球菌的对虾的存活率。进一步研究发现MjCru I-1能增强血细胞对鳗弧菌和沙门氏菌的吞噬作用。金黄色。这些结果表明，MjCru I-1在对虾的抗菌免疫中具有杀菌和促进吞噬作用的双重功能。(C)2014爱思唯尔有限公司版权所有。
Crustins are cationic cysteine-rich antimicrobial peptides (AMPS) that contain multiple domains (glycine-rich, cysteine-rich, or proline-rich) at the N-terminus and whey acidic protein (WAP) domains at the C-terminus. Crustins haVe multiple functions, including protease inhibition and antimicrobial activity. Other functions of crustins need to be clarified. In this study, a novel crustin with a cysteine-rich region, and a single WAP domain, belonging to type I crustins, was identified in Marsupenaeus japonicus and designated as MjCru I-1. MjCru I-1 was expressed in various tissues. The expression of MjCru I-1 was upregulated in the hemocytes of shrimp challenged with bacteria. MjCru I-1 could bind to bacteria by binding to the cell wall molecules of the bacteria, such as lipopolysaccharide (LPS), peptidoglycan (PGN), and lipoteichoic acid (LTA). The synthesized WAP domain of MjCru I-1 but not synthesized Cys-rich domain has antibacterial and agglutinative activities. Scanning electron microscope assay showed that the bacterial cells treated with sMjCru I-1 appeared to be disrupted and cracked compared with those of the control samples. The knockdown of MjCru I-1 could reduce bacterial clearance and injection of MjCru I-1 could significantly increase the survival rate of shrimp infected with Vibrio anguillarum and Staphylococcus aureus compared with those of the control samples. Further study discovered that MjCru I-1 could increase the hemocyte phagocytosis against V. anguillarum and S. aureus. These results suggest that MjCru I-1 has dual functions, bactericidal and phagocytosis promoting activities, in the antibacterial immunity of shrimp. (C) 2014 Elsevier Ltd. All rights reserved.