Initial analysis of copy number variations in cattle selected for resistance or susceptibility to intestinal nematodes
Initial analysis of copy number variations in cattle selected for resistance or susceptibility to intestinal nematodes
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DOI：
10.1007/s00335-010-9308-0
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发表时间：
2011-02-01
期刊：
影响因子：
2.5
通讯作者：
Gasbarre, Louis C.
中科院分区：
文献类型：
--
作者：
Liu, George E.;Brown, Twain;Gasbarre, Louis C.
基因组结构变异是遗传和表型变异的重要来源。我们报告的初步分析的拷贝数变异（CNVs）在牛选择的抗性或敏感性肠道线虫。我们进行了三个阵列比较基因组杂交（CGH）实验，比较安格斯牛的粪便鸡蛋数和血清胃蛋白酶原水平的极端表型。我们总共鉴定了20个CNV，其中12个在已知的染色体内，携带或邻近获得或丢失。约85%（17/20）的CNV与最近报道的牛CNV区域重叠。通过使用定量PCR（qPCR）和FISH的独立方法进一步验证选择的CNV。通路分析表明，这些可变区内的注释牛基因特别富集影响受体活性、信号转导和转录的免疫功能。对差异表达基因启动子区域内的转录因子结合位点（TFBS）的分析表明，共同的转录因子可能参与了寄生虫的抗性。这些结果提供了有价值的假设，为未来的研究牛CNVs下的经济重要的健康和生产性状。
Genomic structural variation is an important and abundant source of genetic and phenotypic variation. We report an initial analysis of copy number variations (CNVs) in cattle selected for resistance or susceptibility to intestinal nematodes. We performed three array comparative genomic hybridization (CGH) experiments to compare Angus cattle with extreme phenotypes for fecal egg count and serum pepsinogen level. We identified 20 CNVs in total, of which 12 were within known chromosomes harboring or adjacent to gains or losses. About 85% of the CNV identified (17/20) overlapped with cattle CNV regions that were reported recently. Selected CNVs were further validated by independent methods using quantitative PCR (qPCR) and FISH. Pathway analyses indicated that annotated cattle genes within these variable regions are particularly enriched for immune function affecting receptor activities, signal transduction, and transcription. Analysis of transcription factor binding sites (TFBS) within the promoter regions of differentially expressed genes suggested that common transcription factors are probably involved in parasite resistance. These results provide valuable hypotheses for the future study of cattle CNVs underling economically important health and production traits.