Graphene oxide size-dependently altered lipid profiles in THP-1 macrophages
Graphene oxide size-dependently altered lipid profiles in THP-1 macrophages
复制标题
THP-1 巨噬细胞中氧化石墨烯尺寸依赖性改变的脂质谱
DOI：
10.1016/j.ecoenv.2020.110714
复制
发表时间：
2020-08-01
影响因子：
6.8
通讯作者：
Cao, Yi
中科院分区：
文献类型：
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作者：
Luo, Yingmei;Peng, Jinfeng;Cao, Yi
以往的研究主要集中在氧化石墨烯(GO)与巨噬细胞的生物相容性上，但对GO对巨噬细胞脂类的影响研究较少。在这里，我们研究了THP-1巨噬细胞与不同大小的GO(500-5000 nm的GO，记为GO-L；500 nm的GO，记为GO-S)的相互作用。结果发现，暴露24 h后，GO的内化作用似乎很小，而GO-L而不是GO-S可减少脂质堆积，并显著减少可溶性单核细胞趋化蛋白-1(MCP-1)的释放，但不影响白介素6(IL-6)的释放。此外，脂质组学数据显示，GO-L降低了17种脂类的水平，而GO-S仅降低了5种脂类的水平。相比之下，50微克/毫升的炭黑(CB)显著增加了脂质的积累，并有相当大的颗粒内在化。GO-L减少脂质积累与增加ROS或诱导自噬无关，化学物质对自噬的调节也不能改变GO-GO-GO对脂质积累的影响。然而，GO暴露降低了与脂滴生物发生相关的过氧化体增殖物激活受体信号通路中关键成分的基因和蛋白水平，化学物质对PPARγ的调节改变了GO-L对脂质积累的影响。综上所述，我们的结果提示GO对THP-1巨噬细胞脂类成分的影响具有大小依赖性，这可能与PPAR信号通路有关。
Previous studies focused on biocompatibility of graphene oxide (GO) to macrophages, but the impact of GO on lipid profiles in macrophages was less investigated. Herein, we investigated the interactions between THP-1 macrophages and GO of different sizes (GO of size 500-5000 nm, denoted as GO-L; GO of size < 500 nm, denoted as GO-S). We found that after 24 h exposure, the internalization of GO appeared to be minimal, whereas up to 50 mu g/mL of GO-L but not GO-S reduced lipid accumulation, accompanying with a significantly reduced release of soluble monocyte chemoattractant protein-1 (MCP-1) but not interleukin-6 (IL-6). Moreover, lipidomic data showed that GO-L decreased the levels of 17 lipid classes, whereas GO-S only decreased the levels of 5 lipid classes. For comparison, 50 mu g/mL carbon black (CB) significantly increased lipid accumulation with considerable particle internalization. GO-reduced lipid accumulation was not related with increase of reactive oxygen species (ROS) or induction of autophagy, and modulation of autophagy by chemicals showed no significant effect to alter the effects of GO-L on lipid accumulation. However, exposure to GO reduced the mRNA and protein levels of key components in peroxisome proliferators-activated receptor (PPAR) signaling pathway, a pathway that is related with lipid droplet biogenesis, and the modulation of PPAR gamma by chemicals altered the effects of GO-L on lipid accumulation. In conclusion, our results suggested that GO size-dependently altered lipid profiles in THP-1 macrophages that might be related with PPAR signaling pathway.