Dynamic of plasma IL-22 level is an indicator of thymic output after allogeneic hematopoietic cell transplantation
Dynamic of plasma IL-22 level is an indicator of thymic output after allogeneic hematopoietic cell transplantation
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血浆IL-22水平动态是异基因造血细胞移植后胸腺输出的指标
DOI：
10.1016/j.lfs.2020.118849
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发表时间：
2021-01-15
期刊：
影响因子：
6.1
通讯作者：
Pan，Bin
中科院分区：
文献类型：
--
作者：
Shang，Longmei;Duah，Maxwell;Pan，Bin
白细胞介素-22（IL-22）在临床前异基因造血细胞移植模型中促进胸腺恢复和改善T细胞恢复。然而，IL-22和胸腺恢复之间的相关性是未知的，在人类transplant.Materials和MethodsIn这项研究中，血浆IL-22水平的移植人进行了分析围移植。通过检测血液信号联合T细胞受体切除环（TRECs）来评估胸腺输出。流式细胞仪检测移植后14天和28天血浆IL-22水平与外周血TRECs水平呈正相关。多元线性回归分析显示，血浆IL-22水平、急性移植物抗宿主病（aGVHD）发生率和年龄与移植后第28天血TRECs水平显著相关。在第14天和第28天期间血浆IL-22水平的增加与同种异体移植受者中血液TRECs和幼稚T细胞水平的更快恢复相关。根据其TREC水平的中位数分裂，与具有低TREC水平的那些相比，在第28天具有高TREC水平的受体具有较低的aGVHD发生率，在高IL-22水平和低IL-22水平组群中也观察到这种效果。血浆IL-22水平的动态变化是胸腺输出量和aGVHD发生的一个指标。监测血浆IL-22水平有助于评估同种异体移植胸腺功能的恢复情况。
AimsInterleukin-22 (IL-22) promotes thymus recovery and improves T-cell recovery in preclinical allogeneic hematopoietic cell transplant models. However, the correlation between IL-22 and thymus recovery is unknown in human transplant.Materials and methodsIn this study, plasma IL-22 levels of transplanted humans were analyzed peri-transplant. Thymic output was assessed by detecting blood signal joint T-cell receptor excision circles (TRECs). Flow cytometry was applied to measure T-cell subsets.Key findingsPlasma IL-22 level positively correlated with blood TRECs level at days 14 and 28 posttransplant. Multiple linear regression analysis showed plasma IL-22 level, occurrence of acute graft-versus-host disease (aGVHD) and age were significantly associated with blood TRECs level at day 28 after allotransplant. An increase of plasma IL-22 level during day 14 and day 28 correlated with faster recovery of blood TRECs and naïve T-cell levels in allotransplant recipients. Recipients with high TRECs levels at day 28 had lower incidence of aGVHD comparing with those who with low TRECs levels according to a median split of their TRECs levels, an effect also seen in the high IL-22 level and low IL-22 level cohorts. Other factors such as age and infection had impacts on plasma IL-22 level in allotransplants.SignificanceOur findings suggest that dynamic change of plasma IL-22 level is an indicator of thymic output and occurrence of aGVHD. Monitoring plasma IL-22 level might help to assess recovery of thymus function in human allotransplants.