PROBING SINGLE-MOLECULE DYNAMICS
PROBING SINGLE-MOLECULE DYNAMICS
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DOI：
10.1126/science.265.5170.361
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发表时间：
1994-07-15
期刊：
影响因子：
56.9
通讯作者：
DUNN, RC
中科院分区：
文献类型：
--
作者：
XIE, XS;DUNN, RC
用近场光学方法研究了分散在玻璃表面的磺胺101单分子在两个不同时间尺度上的室温动力学。在10(-2)到10(2)秒的时间尺度上，通过偏振测量来检查单分子发射中的强度波动，从而提供对其光谱性质的洞察。在纳秒时间尺度上，测量了单分子的荧光寿命，并表征了它们的激发态能量向近场探头铝层的转移。一部时间分辨发射的电影证明了荧光寿命成像的可行性，它具有单分子灵敏度、皮秒时间分辨率和超过衍射极限的空间分辨率。
The room temperature dynamics of single sulforhodamine 101 molecules dispersed on a glass surface are investigated on two different time scales with near-field optics. On the 10(-2)- to 10(2)-second time scale, intensity fluctuations in the emission from single molecules are examined with polarization measurements, providing insight into their spectroscopic properties. On the nanosecond time scale, the fluorescence lifetimes of single molecules are measured, and their excited-state energy transfer to the aluminum coating of the near-field probe is characterized. A movie of the time-resolved emission demonstrates the feasibility of fluorescence lifetime imaging with single molecule sensitivity, picosecond temporal resolution, and a spatial resolving power beyond the diffraction limit.