Growth curves and survival characteristics of the animals used in the biomarkers of aging program
Growth curves and survival characteristics of the animals used in the biomarkers of aging program
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DOI：
10.1093/gerona/54.11.b492
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发表时间：
1999-11-01
影响因子：
5.1
通讯作者：
Hart, RW
中科院分区：
文献类型：
--
作者：
Turturro, A;Witt, WW;Hart, RW
国家毒理学研究中心（NCTR）和国家老龄化研究所（NIA）之间的合作机构间协议旨在确定和验证一组衰老啮齿动物的生物标志物，以快速测试旨在减缓衰老的干预措施的有效性和安全性。另一个目标是为开发人类衰老生物标志物提供基础，假设在不同基因型和物种中有用的生物标志物对推断到人类的基本过程敏感。热量限制（CR），唯一的干预措施，一贯延长各种物种的平均寿命和最大寿命，被用来提供一个模型与延长寿命。饲养C57 B1/6 NNia、DBA/2 JNia、B6 D2 F1和B6 C3 F1小鼠以及Brown Norway（BN/RijNia）、Fischer（F344/NNia）和Fischer x Brown Norway杂交（F344 x BN F1）大鼠，并在研究中进行饲养。NCTR从超过60，000只单独圈养的7种不同基因型和两种性别的动物中生成数据，其中约一半动物自由采食（AL），其余动物CR。大约一半的动物一半运往国际非研究中心NIA研究者处，其余大部分动物留在NCTR直至死亡。该合作为研究人员提供了健康，长寿的啮齿动物模型的选择，同时允许在不同的喂养模式下开发这些基因型的生命剥落，食物消耗和生长特征的一些最确定的信息。
The collaborative Interagency Agreement between the National Center for Toxicological Research (NCTR) and the National Institute on Aging (NIA) was aimed at identifying and validating a panel of biomarkers of aging bl rodents ill order to rapidly test the efficacy and safety of interventions designed to slow aging. Another aim was to provide a basis for developing biomarkers of aging in humans, using the assumption that biomarkers that were useful across different genotypes and species were sensitive to fundamental processes that would extrapolate to humans. Caloric restriction (CR), the only intervention that consistently extends both mean and maximal life span in a variety of species, was used to provide a model with extended life span. C57B1/6NNia, DBA/2JNia, B6D2F1, and B6C3F1 mice and Brown Norway (BN/RijNia), Fischer (F344/NNia) and Fischer x Brown Norway hybrid (F344 x BN F1) rats were bred and maintained on study. NCTR generated data from over 60,000 individually housed animals of the seven different genotypes and both sexes, approximately half ad libitum (AL) fed, the remainder CR. Approximately half the animals half shipped to offsite NIA investigators internationally with the majority of the remainder maintained at NCTR until they died. The collaboration supplied a choice of healthy, long-lived rodent models to investigators, while allowing for the development of some of the most definitive information on life spall, food consumption, and growth characteristics in these genotypes under diverse feeding paradigms.