All 16 centromere DNAs from Saccharomyces cerevisiae show DNA curvature
All 16 centromere DNAs from Saccharomyces cerevisiae show DNA curvature
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DOI：
10.1093/nar/27.6.1444
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发表时间：
1999-03-15
影响因子：
14.9
通讯作者：
Hegemann, JH
中科院分区：
文献类型：
--
作者：
Bechert, T;Heck, S;Hegemann, JH
克隆了酿酒酵母（Saccharomycescerevisiae）的16个着丝粒DNA区域，包括两侧各90 bp的框架序列。用聚丙烯酰胺凝胶电泳分析了这些300 bp长的着丝粒区域，发现它们的迁移率降低，表明DNA的弯曲程度与着丝粒有关。实验结果与计算机分析的三维结构相吻合，进一步证实了CEN DNA结构对着丝粒蛋白-DNA复合物的组装、活性和/或调控的重要性。
All 16 centromere DNA regions of Saccharomyces cerevisiae including 90 bp framing sequences on either side were cloned, These 300 bp long centromere regions were analysed by native polyacrylamide gel electrophoresis and found to display a reduced mobility indicative of DNA curvature, The degree of curvature is centromere dependent. The experimental data were confirmed by computer analysis of the 3-dimensional structure of the CEN DNAs, Altogether these data provide further evidence for a model for budding yeast centromeres in which CEN DNA structure could be important for the assembly, activity and/or regulation of the centromere protein-DNA complex.