Identification and characterization of microRNAs in the white-backed planthopper, Sogatella furcifera
Identification and characterization of microRNAs in the white-backed planthopper, Sogatella furcifera
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白背飞虱 (Sogatellafurcifera) 中 microRNA 的鉴定和表征。
DOI：
10.1111/1744-7917.12343
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发表时间：
2016-06-01
期刊：
影响因子：
4
通讯作者：
Wu, Qing-Fa
中科院分区：
文献类型：
--
作者：
Chang, Zhao-Xia;Tang, Nan;Wu, Qing-Fa
微小RNA（microRNAs，miRNAs）是一类新型的内源性非编码小RNA，在真核生物的多种代谢活动中起着重要的调控作用。白背飞虱是南方水稻黑条矮缩病毒（SRBSDV）唯一的传播媒介，目前关于白背飞虱中miRNAs的鉴定报道有限。在这项研究中，S.利用高通量测序技术，从含病毒和不含病毒的S. furcifera的两个小RNA文库中获得了106个保守的和276个新的miRNA。这些miRNA分别属于52个保守的miRNA家族和58个S. furcifera特有的家庭，分别。与miRBase中26种昆虫和其他5种昆虫的miRNAs比较发现，超过一半的miRNAs家族在六足类中高度保守，而其他miRNAs家族仅在非双翅目中高度保守。此外，通过Gene Ontology对382个miRNAs进行功能分类，得到4117个靶基因。与未感染病毒的细胞相比，在感染SRBSDV的细胞中发现了8个上调的miRNAs和4个下调的miRNAs，其中miR-14和miR-n98 a可能参与了SRBSDV感染的免疫应答。对这些miRNAs的分析将有助于深入了解这些miRNAs在代谢、发育和病毒感染相关基因的调控和表达中的作用。furcifera。
MicroRNAs (miRNAs) are a novel class of small, non-coding endogenous RNAs that play critical regulatory roles in many metabolic activities in eukaryotes. Reports of the identification of miRNAs in Sogatella furcifera (white-backed planthopper), the insect that acts as the only confirmed vector of the southern rice black-streaked dwarf virus (SRBSDV), are limited. In this study, a total of 382 miRNAs were identified in S. furcifera, including 106 conserved and 276 novel miRNAs, using high-throughput sequencing based on two small RNA libraries from viruliferous and non-viruliferous S. furcifera, and these miRNAs belonged to 52 conserved miRNA families and 58 S. furcifera-specific families, respectively. Comparison with miRNAs from 26 insect species and five other species in miRBase showed that more than half of the conserved miRNA families are highly conserved in Hexapoda, while othermiRNAs are only conserved in non-dipterans. Furthermore, 4 117 target genes predicted for the 382 identified miRNAs could be categorized into 45 functional groups annotated by Gene Ontology. Compared with non-viruliferous cells, eight up-regulated miRNAs and four down-regulated miRNAs were identified in cells inoculated with SRBSDV, among which miR-14 and miR-n98a may be involved in the immune response to SRBSDV infection. Analyses of the identified miRNAs will provide insights into the roles of these miRNAs in the regulation and expression of genes involved in the metabolism, development and viral infection of S. furcifera.