EFFECT OF SALT REGIMENS ON DEVELOPMENT OF (NA+-5+)- DEPENDENT ATPASE ACTIVITY DURING GROWTH OF SALT GALNDS OF DUCKLINGS
EFFECT OF SALT REGIMENS ON DEVELOPMENT OF (NA+-5+)- DEPENDENT ATPASE ACTIVITY DURING GROWTH OF SALT GALNDS OF DUCKLINGS
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DOI：
10.1016/0005-2736(67)90098-3
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发表时间：
1967-01-01
期刊：
影响因子：
--
通讯作者：
ELLIS, RA
中科院分区：
文献类型：
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作者：
ERNST, SA;GOERTEMI.CC;ELLIS, RA
研究了家鸭盐腺匀浆中Mg2+激活型、瓦阿巴因敏感型(Na+-K+)- atp酶和瓦阿巴因不敏感型Mg2+ - atp酶的特征。孵化后第3天，给予实验鸟1% NaCl水溶液饮用12小时。每天剩下的时间都有淡水。对照组的雏鸭只喝新鲜的自来水。在实验鸟盐腺匀浆中，atp酶的比活性在第9天达到最高水平。（Na+ -K+） - atp酶增加了4.1倍，Mg2+- atp酶增加了1.8倍。对照鸟的匀浆中atp酶活性没有增加。仅饮用淡水的鸟类腺体均质液在盐水环境下维持22天后，(Na+-K+)- atp酶活性呈对数下降，并在9天后达到仅饮用淡水的对照鸟的活性水平。Mg2+- atp酶活性在淡水处理前5天下降较慢，在试验后4天迅速下降。在盐水方案中，鸟类盐腺中atp酶水平的增加并不是阳离子特异性的：KC1和MgCl2方案都能够增加这一水平，尽管两者都不如NaCl有效。不同盐饮食导致的(Na+-K+)- atp酶和Mg2+ - atp酶活性的不同增减率表明，这些活性可能不是同一酶双峰功能的结果。
Mg2+ -activated, ouabain-sensitive (Na+-K+)-ATPase and a ouabain-insensitive Mg2+ -ATPase were characterized in salt gland homogenates of the domestic duck. Thrre days after hatching, experimental birds were given 1% aqueous NaCl to drink for 12 hr. and fresh water for the remainder of each day. Control ducklings were maintained exclusively on fresh tap water. In homogenates of salt glands from experimental birds the specific activity of ATPase reached maximal levels in 9 days. (Na+ -K+) -ATPase increased by a factor 4.1 and Mg2+-ATPase increased by a factor of 1.8. There was no increase in ATPase activity in homogenates from control birds. Homogenates of glands from birds drinking only fresh water, after being maintained on the salt-water regimen for 22 days, exhibited a logarithmic decrease in (Na+-K+)-ATPase activity and in 9 days reached the level of activity recorded for control birds maintained only on fresh water. The Mg2+-ATPase activity decreased rather slowly for the 1st 5 days on fresh water, and then dropped rapidly during the last 4 days of the experiment. The increase in ATPase levels in the salt glands of birds on the salt-water regimen is not cation-specific: both KC1 and MgCl2 regimens are capable of increasing this level, although neither is as effective as NaCl. Differential rates of increase and decrease of (Na+-K+)-ATPase and Mg2+ -ATPase activity resulting from various salt diets suggest that these activities may not be a consequence of bimodal functioning of the same enzyme.