Multi-omics analysis reveals the host-microbe interactions in aged rhesus macaques.
Multi-omics analysis reveals the host-microbe interactions in aged rhesus macaques.
复制标题
多组学分析揭示老年恒河猴宿主与微生物的相互作用
DOI：
10.3389/fmicb.2022.993879
复制
发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
衰老是一个复杂的多因素过程，极大地影响动物健康。多组学分析广泛应用于进化生物学和生物医学研究。然而，多组学是否能够提供足够的信息来揭示老年非人灵长类动物的全面变化仍不清楚。在这里，我们利用多组学分析探讨了中国恒河猴（Macaca mulatta lasiota，CRs）衰老过程中宿主-微生物相互作用的变化。结果显示，年轻 CR 和老年 CR 之间的口腔和肠道微生物群发生显着变化，包括老年 CR 中益生菌丰度显着降低和致病菌丰度增加。值得注意的是，可以代谢色氨酸产生芳基烃受体（AhR）配体的乳酸菌的丰度在老化的 CR 中减少。代谢组学一致检测到 AhR 配体血浆水平下降。此外，老年CR中游离脂肪酸、酰基肉碱、肝素、2-(4-羟苯基)丙酸和二十二碳六烯酸乙酯水平升高，这可能导致脂肪酸代谢异常和心血管疾病。转录组分析确定了与色氨酸代谢和炎症相关的基因表达的变化。总之，发现了不同组学之间的许多潜在联系，表明老年 CR 面临多种代谢问题、免疫紊乱以及口腔和肠道疾病。我们确定色氨酸代谢对于老年 CR 的生理健康至关重要。我们的研究结果证明了多组学分析在揭示非人类灵长类动物宿主-微生物相互作用方面的价值，并表明类似的方法可以应用于其他物种的进化和生态研究。
Aging is a complex multifactorial process that greatly affects animal health. Multi-omics analysis is widely applied in evolutionary biology and biomedical research. However, whether multi-omics can provide sufficient information to reveal comprehensive changes in aged non-human primates remains unclear. Here, we explored changes in host–microbe interactions with aging in Chinese rhesus macaques (Macaca mulatta lasiota, CRs) using multi-omics analysis. Results showed marked changes in the oral and gut microbiomes between young and aged CRs, including significantly reduced probiotic abundance and increased pathogenic bacterial abundance in aged CRs. Notably, the abundance of Lactobacillus, which can metabolize tryptophan to produce aryl hydrocarbon receptor (AhR) ligands, was decreased in aged CRs. Consistently, metabolomics detected a decrease in the plasma levels of AhR ligands. In addition, free fatty acid, acyl carnitine, heparin, 2-(4-hydroxyphenyl) propionic acid, and docosahexaenoic acid ethyl ester levels were increased in aged CRs, which may contribute to abnormal fatty acid metabolism and cardiovascular disease. Transcriptome analysis identified changes in the expression of genes associated with tryptophan metabolism and inflammation. In conclusion, many potential links among different omics were found, suggesting that aged CRs face multiple metabolic problems, immunological disorders, and oral and gut diseases. We determined that tryptophan metabolism is critical for the physiological health of aged CRs. Our findings demonstrate the value of multi-omics analyses in revealing host–microbe interactions in non-human primates and suggest that similar approaches could be applied in evolutionary and ecological research of other species.