CLONAL ANALYSIS OF THE TISSUE-SPECIFICITY OF RECESSIVE FEMALE-STERILE MUTATIONS OF DROSOPHILA-MELANOGASTER USING A DOMINANT FEMALE-STERILE MUTATION FS(1)K1237
CLONAL ANALYSIS OF THE TISSUE-SPECIFICITY OF RECESSIVE FEMALE-STERILE MUTATIONS OF DROSOPHILA-MELANOGASTER USING A DOMINANT FEMALE-STERILE MUTATION FS(1)K1237
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DOI：
10.1016/0012-1606(83)90231-2
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发表时间：
1983-01-01
影响因子：
2.7
通讯作者：
GANS, M
中科院分区：
文献类型：
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作者：
PERRIMON, N;GANS, M
利用新分离的生殖系依赖显性雌性不育突变Fs（1）K1237，我们鉴定了25个X连锁隐性雌性不育突变的生殖系或体细胞系依赖性。由于Fs（1）K1237+雌性不能产卵，只有由于X射线诱导的有丝分裂重组而失去Fs（1）K1237的生殖系细胞才能产生卵子。这样的重组事件将使同源染色体上的基因纯合。如果这条染色体携带隐性雌性不育突变，只有在生殖系不需要改变功能的情况下，才能恢复生育能力。使用这个测试，我们已经分类25隐性雌性不育突变：12影响生殖系功能，12影响体细胞系功能，并给出了一个模糊的结果，提出了解释。对于一些体细胞系依赖的突变体，我们发现一些来自生殖系克隆的卵表现出与隐性雌性不育突变纯合子的雌性所产的卵相同的表型。这些结果进行了讨论的一致生产的克隆在卵泡细胞。
Using the newly isolated, germ line-dependent dominant female-sterile mutation Fs (1) K1237, we have characterized the germ line or somatic line dependence of 25 X-linked recessive female-sterile mutations. Since Fs (1) K1237+ females fail to lay eggs, only germ line cells which lose Fs (1) K1237 as a result of X-ray-induced mitotic recombination are capable of producing eggs. Such recombination events will render genes on the homologous chromosome homozygous. If this chromosome carries a recessive female-sterile mutation, the fertility will be restored only if the altered function is not required in the germ line. Using this test, we have classified 25 recessive female-sterile mutations: 12 affect germ line function, 12 affect somatic line function, and one gave an ambiguous result for which an explanation is proposed. For a few of the somatic line-dependent mutants, we found that some eggs derived from germ line clones showed the same phenotype as eggs laid by females homozygous for the recessive female-sterile mutation. These results are discussed in terms of a coincident production of clones in the follicle cells.