A Loop-Mediated Isothermal Amplification Assay Targeting Neisseria Gonorrhoeae penA-60.001
A Loop-Mediated Isothermal Amplification Assay Targeting Neisseria Gonorrhoeae penA-60.001
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针对淋病奈瑟菌 penA-60.001 的环介导等温扩增测定
DOI：
10.1128/aac.01663-19
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Makoto Ohnishi
中科院分区：
文献类型：
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作者：
Ken Shimuta;Shu-Ichi Nakayama;Hideyuki Takahashi;Makoto Ohnishi
头孢曲松（CRO）被广泛用作淋球菌感染的一线治疗药物。然而，最近出现了携带mosaicpenA-60.001的抗CRO淋球菌菌株，并在世界范围内传播。为了满足检测这些菌株的迫切需要，我们在这里报告了一个环介导等温扩增（LAMP）检测系统，目标是N。gonorrhoeaepenA-60.001.该检测系统可以区分N.携带mosaicpenA-60.001的淋球菌菌株与携带其他等位基因的淋球菌菌株之间的差异。
Ceftriaxone (CRO) is widely used as the first-line treatment for gonococcal infections. However, CRO-resistant Neisseria gonorrhoeae strains carrying mosaicpenA-60.001 have emerged recently and disseminated worldwide. To meet the urgent need to detect these strains, we report here a loop-mediated isothermal amplification (LAMP) assay system that targets N. gonorrhoeaepenA-60.001. This assay system can differentiate N. gonorrhoeae strains carrying mosaicpenA-60.001 from strains carrying otherpenAalleles.