THE ROLE OF SWI4 AND SWI6 IN THE ACTIVITY OF G1 CYCLINS IN YEAST
THE ROLE OF SWI4 AND SWI6 IN THE ACTIVITY OF G1 CYCLINS IN YEAST
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DOI：
10.1016/0092-8674(91)90444-4
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发表时间：
1991-09-06
期刊：
影响因子：
64.5
通讯作者：
DIRICK, L
中科院分区：
文献类型：
--
作者：
NASMYTH, K;DIRICK, L
进入有丝分裂周期(START)需要由CDC28基因编码的蛋白激酶和由CLN1、-2和-3编码的三个冗余的G1特定周期蛋白之一。SWI4和SWI6是HO内切酶基因启动依赖激活所必需的转录因子。它们也完成了重叠但对细胞分裂至关重要的作用，因为这两个基因缺失的细胞是不能存活的。我们发现SWI4和SWI6的重要作用是确保G1特异性细胞周期蛋白基因的活性。SWI4和SWI6似乎是CLN1和CLN2转录所必需的，而不是CLN3转录所必需的。然而，CLN3功能也依赖于SWI4和SWI6。
Entry into the mitotic cycle (START) requires a protein kinase encoded by the CDC28 gene and one of three redundant G1-specific cyclins encoded by CLN1, -2, and -3. SWI4 and SWI6 are transcription factors required for the START-dependent activation of the HO endonuclease gene. They also fulfill an overlapping but essential role for cell division since cells deleted for both genes are inviable. We show that the essential role of SWI4 and SWI6 is to ensure the activity of G1-specific cyclin genes. SWI4 and SWI6 appear necessary for the transcription of CLN1 and CLN2, but not for that of CLN3. CLN3 function is, however, also dependent on SWI4 and SWI6.