A comparative analysis of human adult testicular cells expressing stem Leydig cell markers in the interstitium, vasculature, and peritubular layer
A comparative analysis of human adult testicular cells expressing stem Leydig cell markers in the interstitium, vasculature, and peritubular layer
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DOI：
10.1111/andr.12817
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发表时间：
2020-06-01
期刊：
影响因子：
4.5
通讯作者：
van Pelt, Ans M. M.
中科院分区：
文献类型：
--
作者：
Eliveld, Jitske;van Daalen, Saskia K. M.;van Pelt, Ans M. M.
背景人类成年间质细胞(ALCs)的起源尚不清楚。目的本研究旨在探讨人(PDGFRα，NGFR)和啮齿动物(NES，PDGFRα，THY1，NR2F2)SLC标志物在体内和体外培养前后在成人睾丸间质细胞中的表达情况。材料与方法应用免疫组织化学方法对表达SLC标志物的成人睾丸间质细胞进行定位。然后，分离培养间质细胞。结果NR2F2和PDGFRα表达于肾小管周围、血管周围和间质细胞，而THY1表达于肾小管周围和血管周围细胞。尽管NES和NGFR在内皮细胞中表达，但在体外两者都与PDGFRα共同定位，尽管NGFR仅在培养后才表达。结论PDGFRα、NR2F2和THY1在人睾丸细胞中的定位和表达部分重叠，表明PDGFRα、NR2F2和THY1在SLC发育的同一谱系中表达。基于体外结果，NES和NGFR体外扩增后也是如此。结论SLC标志物在重叠的人间质细胞群中表达，为进一步研究Leydig细胞谱系开辟了更好的研究策略，并将有助于开发治疗ALC功能障碍的策略。
Background Origin of human adult Leydig cells (ALCs) is not well understood. This might be partly due to limited data available on the identification and location of human precursor and stem Leydig cells (SLCs) which hampers the study on the development of ALCs.Objectives The aim of the present study was to investigate whether described human (PDGFR alpha, NGFR) and rodent (NES, PDGFR alpha, THY1, NR2F2) SLC markers are expressed by a common cell population within human adult testicular interstitial cells in vivo and before and after in vitro propagation.Materials and methods Immunohistochemical analyses were used to identify localization of human adult testicular interstitial cells expressing described SLC markers. Next, interstitial cells were isolated and cultured. The percentage of cells expressing one or more SLC markers was determined before and after culture using flow cytometry.Results NR2F2 and PDGFR alpha were present in peritubular, perivascular, and Leydig cells, while THY1 was expressed in peritubular and perivascular cells. Although NES and NGFR were expressed in endothelial cells, co-localization with PDGFR alpha was found for both in vitro, although for NGFR only after culture. All marker positive cells were able to undergo propagation in vitro.Discussion The partly overlap in localization and overlap in expression in human testicular cells indicate that PDGFR alpha, NR2F2, and THY1 are expressed within the same ALC developmental lineage from SLCs. Based on the in vitro results, this is also true for NES and after in vitro propagation for NGFR.Conclusion Our results that earlier described SLC markers are expressed in overlapping human interstitial cell population opens up further research strategies aiming for a better insight in the Leydig cell lineage and will be helpful for development of strategies to cure ALC dysfunction.