T-CELL CD3-ZETA-ETA HETERODIMER EXPRESSION AND COUPLING TO PHOSPHOINOSITIDE HYDROLYSIS
T-CELL CD3-ZETA-ETA HETERODIMER EXPRESSION AND COUPLING TO PHOSPHOINOSITIDE HYDROLYSIS
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DOI：
10.1126/science.2845582
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发表时间：
1988-10-28
期刊：
影响因子：
56.9
通讯作者：
ASHWELL, JD
中科院分区：
文献类型：
--
作者：
MERCEP, M;BONIFACINO, JS;ASHWELL, JD
T细胞抗原受体由抗原结合异二聚体组成，所述抗原结合异二聚体与至少五个CD 3亚基（γ，δ，ε，. ζ，和η）。CD3-ζ链是二硫键连接的同二聚体（CD 3-ζ 2）或具有η的二硫键连接的异二聚体。（CD3-ζ）eta.）。表达正常量的CD 3-ζ 2但降低量的CD 3-ζ的鼠抗原特异性T细胞杂交瘤的变体。式-η中分离出当激活时，亲本细胞系增加磷脂酰肌醇水解和丝氨酸特异性蛋白激酶活性的程度比变体大得多。与此相反，激活后的酪氨酸特异性激酶的刺激与交联抗体的CD 3是相似的这些细胞。CD 3-ζ的表达之间存在正线性关系。式-η和由TCR刺激的磷酸肌醇水解，表明T细胞α的差异偶联。β的异源二聚体的信号转导机制。β的与CD 3-ζ 2或CD 3-ζ结合。式-η
The T cell antigen receptor consists of an antigen-binding heterodimer that is noncovalently associated with at least five CD3 subunits (.gamma., .delta., .epsilon., .zeta., and .eta.). The CD3-.zeta. chains are either disulfide-linked homodimers (CD3-.zeta.2) or disulfide-linked heterodimers with .eta. (CD3-.zeta..eta.). Variants of a murine antigen-specific T cell hybridoma that express normal amounts of CD3-.zeta.2 but decreased amounts of CD3-.zeta..eta. were isolated. When activated, the parental cell line increased both phosphatidylinositol hydrolysis and serine-specific protein kinase activtiy to a much greater extent than the variants. In contrast, the activation of a tyrosine-specific kinase after stimulation with a cross-linking antibody to CD3 was similar among these cells. There was a positive linear relation between the expression of CD3-.zeta..eta. and phosphoinositide hydrolysis stimulated by the TCR, suggesting a differential coupling of the T cell .alpha..beta. heterodimer to signal transduction mechanisms due to .alpha..beta. association with either CD3-.zeta.2 or CD3-.zeta..eta.