High circ-SEC31A expression predicts unfavorable prognoses in non-small cell lung cancer by regulating the miR-520a-5p/GOT-2 axis
High circ-SEC31A expression predicts unfavorable prognoses in non-small cell lung cancer by regulating the miR-520a-5p/GOT-2 axis
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circ-SEC31A 高表达通过调节 miR-520a-5p/GOT-2 轴预测非小细胞肺癌的不良预后
DOI：
10.18632/aging.103264
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发表时间：
2020-06-15
期刊：
影响因子：
5.2
通讯作者：
Huang, Gang
中科院分区：
文献类型：
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作者：
Jin, Mingming;Shi, Chunzi;Huang, Gang
近年来研究表明，环状RNA（circRNA）的异常调控在包括非小细胞肺癌（NSCLC）在内的多种肿瘤的发生、发展过程中起着重要的调控作用。然而，大多数circRNA在NSCLC中的作用仍然未知。在这项研究中，我们发现hsa_circ_0001421（circ-SEC 31 A）在NSCLC组织和细胞系中上调。circ-SEC 31 A在NSCLC中的表达增加与恶性特征显著相关，并且是NSCLC患者手术后总生存的独立危险因素。NSCLC中circ-SEC 31 A表达减少可降低肿瘤细胞增殖、迁移、侵袭和苹果酸-天冬氨酸代谢。从机制上讲，我们证明了沉默circ-SEC 31 A通过减轻miR-520 a-5 p的海绵效应来下调GOT-2表达，这导致NSCLC细胞中苹果酸-天冬氨酸代谢显著降低。综上所述，这些结果揭示了circ-SEC 31 A在NSCLC细胞增殖、迁移、侵袭和代谢调节中的重要作用，为circRNA在NSCLC进展中的作用提供了新的视角。
Dysregulation of circular RNAs (circRNAs) has recently been shown to play important regulatory roles in cancer development and progression, including non-small cell lung cancer (NSCLC). However, the roles of most circRNAs in NSCLC are still unknown. In this study, we found that hsa_circ_0001421 (circ-SEC31A) was upregulated in NSCLC tissues and cell lines. Increased circ-SEC31A expression in NSCLC was significantly correlated with malignant characteristics and served as an independent risk factor for the post-surgical overall survival of NSCLC patients. Reduced circ-SEC31A expression in NSCLC decreased tumor cell proliferation, migration, invasion, and malate-aspartate metabolism. Mechanistically, we demonstrated that silencing circ-SEC31A downregulated GOT-2 expression by relieving the sponging effect of miR-520a-5p, which resulted in significantly reduced malate-aspartate metabolism in NSCLC cells. Taken together, these results revealed the important role of circ-SEC31A in the proliferation, migration, invasion, and metabolic regulation of NSCLC cells, providing a new perspective on circRNAs in NSCLC progression.