Eph/ephrins and N-cadherin coordinate to control the pattern of sympathetic ganglia
Eph/ephrins and N-cadherin coordinate to control the pattern of sympathetic ganglia
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DOI：
10.1242/dev.02662
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发表时间：
2006-12-15
期刊：
影响因子：
4.6
通讯作者：
Kulesa, Paul M.
中科院分区：
文献类型：
--
作者：
Kasemeier-Kulesa, Jennifer C.;Bradley, Roger;Kulesa, Paul M.
以前的研究表明，节段性的神经嵴源性交感神经节的出现作为一个直接的结果，限制神经嵴细胞迁移流通过吻体节的一半。我们最近发现，鸡交感神经节形成的时空模式是一个两阶段的过程。神经嵴细胞横向迁移到背主动脉，然后令人惊讶地在纵向方向上扩散，然后分选成离散的神经节。在这里，我们调查的两个家庭的分子，被认为是调节细胞分选和聚集的功能。通过阻断Eph/肝配蛋白或N-钙粘蛋白功能，我们使用最近开发的矢状切片外植体培养和3D共聚焦延时成像来测量神经嵴细胞迁移行为的变化，从而导致交感神经节形成的改变。我们的研究结果表明，局部抑制性相互作用的神经节间区域内，Eph/ephrins介导的，在神经节的网站，由N-钙粘蛋白介导的粘合剂细胞-细胞接触，协调雕塑离散的交感神经节。
Previous studies have suggested that the segmental pattern of neural-crest-derived sympathetic ganglia arises as a direct result of signals that restrict neural crest cell migratory streams through rostral somite halves. We recently showed that the spatiotemporal pattern of chick sympathetic ganglia formation is a two-phase process. Neural crest cells migrate laterally to the dorsal aorta, then surprisingly spread out in the longitudinal direction, before sorting into discrete ganglia. Here, we investigate the function of two families of molecules that are thought to regulate cell sorting and aggregation. By blocking Eph/ephrins or N-cadherin function, we measure changes in neural crest cell migratory behaviors that lead to alterations in sympathetic ganglia formation using a recently developed sagittal slice explant culture and 3D confocal time-lapse imaging. Our results demonstrate that local inhibitory interactions within inter-ganglionic regions, mediated by Eph/ephrins, and adhesive cell-cell contacts at ganglia sites, mediated by N-cadherin, coordinate to sculpt discrete sympathetic ganglia.