Fully Integrated Self-Powered Electrical Stimulation Cell Culture Dish for Noncontact High-Efficiency Plasmid Transfection
Fully Integrated Self-Powered Electrical Stimulation Cell Culture Dish for Noncontact High-Efficiency Plasmid Transfection
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DOI：
10.1021/acsami.1c16748
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发表时间：
2021-11-24
影响因子：
9.5
通讯作者：
Xu, Yang
中科院分区：
文献类型：
--
作者：
Yuan, Miaomiao;Li, Xiaoying;Xu, Yang
质粒DNA转染哺乳动物细胞被广泛应用于生物医学研究和基因药物递送，但其转染效率低，特别是在原代细胞中的转染效率低，限制了其应用。为了提高质粒转染的效率，已经开发了一种完全集成的自供电电刺激细胞培养皿（SESD），以提供贴壁细胞的自供电电刺激（ES），通过标准的脂质体转染方法显著提高质粒转染到哺乳动物细胞中的效率和细胞存活。从机制上讲，ES可以通过开放钙离子通道安全地增加细胞内钙离子浓度，从而提高质粒转染效率。因此，SESD有潜力成为生物医学研究和药物递送中高效质粒DNA转染的有效平台。
Plasmid DNA transfection of mammalian cells is widely used in biomedical research and genetic drug delivery, but low transfection efficiency, especially in the context of the primary cells, limits its application. To improve the efficiency of plasmid transfection, a fully integrated self-powered electrical stimulation cell culture dish (SESD) has been developed to provide self-powered electrical stimulation (ES) of adherent cells, significantly improving the efficiency of plasmid transfection into mammalian cells and cell survival by the standard lipofectamine transfection method. Mechanistically, ES can safely increase the intracellular calcium concentration by opening calcium-ion channels, leading to a higher efficiency of plasmid transfection. Therefore, SESD has the potential to become an effective platform for high-efficiency plasmid DNA transfection in biomedical research and drug delivery.