Bioimaging of Hyaluronic Acid Derivatives Using Nanosized Carbon Dots
Bioimaging of Hyaluronic Acid Derivatives Using Nanosized Carbon Dots
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DOI：
10.1021/bm300796q
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发表时间：
2012-08-01
影响因子：
6.2
通讯作者：
Hahn, Sei Kwang
中科院分区：
文献类型：
--
作者：
Goh, Eun Ji;Kim, Ki Su;Hahn, Sei Kwang
与广泛使用的半导体量子点相比，荧光纳米碳点（Cdot）是一种新兴的生物成像剂，具有优异的化学惰性和边缘细胞毒性。在这项工作中，我们报告的应用cdots的真实的时间生物成像的目标特异性交付的透明质酸（HA）衍生物。以柠檬酸为原料，在热溶剂中热裂解合成了聚乙二醇（PEG）二胺封端的Cdot。所合成的Cdot在紫外光激发下显示出很强的荧光，其发射特性与激发波长有关。通过Cdot的胺基与HA的羧基之间的酰胺键形成来合成HA-Cdot缀合物。在确认Cdot和HA Cdot缀合物的细胞毒性可忽略不计后，进行体外生物成像，以通过HA受体介导的内吞作用靶向特异性细胞内递送HA-Cdot缀合物。此外，Cdot和HA-Cdot缀合物的体内实时生物成像显示HA-Cdot缀合物向具有丰富HA受体的肝脏的靶向特异性递送。总而言之，我们可以确认HA衍生物作为治疗肝脏疾病的靶向特异性药物递送载体的可行性，以及Cdot作为有前途的生物成像剂的可行性。
Fluorescent nanosized carbon dots (Cdots) are an emerging bioimaging agent with excellent chemical inertness and marginal cytotoxicity in comparison to widely used semiconductor quantum dots. In this work, we report the application of Cdots for real time bioimaging of target specific delivery of hyaluronic acid (HA) derivatives. Polyethylene glycol (PEG) diamine-capped Cdots were synthesized by the pyrolysis of citric acid in a hot solvent The synthesized Cdots showed strong fluorescence under UV excitation with emission properties dependending on the excitation wavelength. HA-Cdot conjugates were synthesized by amide bond formation between amine groups of Cdot and carboxylic groups of HA. After confirmation of the negligible cytotoxicity of Cdots and HA Cdot conjugates, in vitro bioimaging was carried out for target specific intracellular delivery of the HA-Cdot conjugates by HA receptor mediated endocytosis. Furthermore, in vivo real-time bioimaging of Cdots and HA-Cdot conjugates exhibited the target specific delivery of HA-Cdot conjugates to the liver with abundant HA receptors. Taken together, we could confirm the feasibility of HA derivatives as a target specific drug delivery carrier for the treatment of liver diseases and Cdots as a promising bioimaging agent.