Mediators and cytokines in allergic and viral-triggered rhinitis
Mediators and cytokines in allergic and viral-triggered rhinitis
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DOI：
10.2500/aap.2007.28.3017
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发表时间：
2007-07-01
影响因子：
2.8
通讯作者：
Kramer, Matthias F.
中科院分区：
文献类型：
--
作者：
Klemens, Christine;Rasp, Gerd;Kramer, Matthias F.
间歇性过敏性鼻炎和普通感冒是常见的疾病，表现出相似的临床症状。本研究旨在探讨变态反应性鼻炎和病毒性鼻炎急性症状患者鼻液中细胞因子的变化规律。鼻腔分泌物采用免疫吸附分析技术，使用细胞因子小组分析和常规的ELISA。变态反应性患者的嗜酸性粒细胞阳离子蛋白(ECK)、白细胞介素5(IL-5)和类胰蛋白酶水平显著升高。病毒性鼻炎患者的促炎细胞因子(IL-1b、IL-6、IL-7、IL-17)、干扰素(干扰素)、肿瘤坏死因子(TNFi-α)、细胞浸润趋化因子(IL-8、单核细胞趋化蛋白1和巨噬细胞炎性蛋白10)、细胞增殖因子(粒细胞集落刺激因子[G-CSF]和粒细胞巨噬细胞集落刺激因子[GM-CSFI])和弹性蛋白酶水平显著升高。IL-10仅在病毒性鼻炎中检测到。病毒性鼻炎患者IL-4水平显著高于变应性鼻炎患者，IL-5水平显著高于正常对照组。在病毒触发的鼻炎中，我们检测到一种以Th1型细胞因子为主的模式，并带有强有力的促炎介质。反映中性粒细胞和嗜酸性粒细胞免疫反应的因子包括IL-5、IL-8、GM-CSF、ECP和弹性蛋白酶。变应性鼻炎患者的鼻分泌物显示最高浓度的类胰蛋白酶、IL-5和ECP，反映肥大细胞和嗜酸性粒细胞的免疫反应。过敏性鼻炎患者的鼻分泌物IL-4水平不是最高，但病毒性鼻炎患者的鼻分泌物IL-4水平最高。IL-4也可能通过抑制炎性细胞因子的产生而在限制炎症过程中发挥作用。
Intermittent allergic rhinitis and common cold constitute frequent conditions and show similar clinical symptoms. The purpose of this study was to investigate the pattern of cytokines in the nasal fluid of patients with acute symptoms caused by allergic and viral rhinitis. Nasal secretions were analyzed by immunosorbent assay techniques using a cytokine panel assay and routine ELISA. Allergic patients had significantly higher levels of eosinophil cationic protein (ECK interleukin (IL)-5, and tryptase. Significantly elevated concentrations of proinflammatory cytokines (IL-1b, IL-6, IL-7, IL-17, interferon [IFN] gamma, and tumor necrosis factor [TNFI-alpha) as well as chemokines for cellular infiltration (IL-8, monocyte chemoattractant protein 1, and macrophage inflammatory protein 10), factors for cellular proliferation (granulocyte colony-stimulating factor [G-CSF] and granulocyte macrophage colony-stimulating factor [GM-CSFI), and elastase were found in viral rhinitis. IL-10 was only detectable in viral rhinitis. IL-4 was significantly higher in patients with viral rhinitis than allergic rhinitis, and IL-5 was significantly elevated in viral rhinitis compared with controls. In viral-triggered rhinitis, we detected a predominantly Th1-type cytokine pattern with potent proinflammatory mediators. Factors reflecting a neutrophil and eosinophil immune response, due to IL-5, IL-8, GM-CSF, ECP, and elastase were shown. Nasal secretions of patients with allergic rhinitis showed highest concentrations of tryptase, IL-5, and ECP, reflecting a mast cell and eosinophil immune response. Nasal secretion levels of IL-4 did not show highest levels in allergic rhinitis but did in viral rhinitis. IL-4 also may play a role in limiting inflammatory processes by inhibiting the production of inflammatory cytokines.