METABOLIC COOPERATION IN ORAL MICROBIAL COMMUNITIES DURING GROWTH ON MUCIN
METABOLIC COOPERATION IN ORAL MICROBIAL COMMUNITIES DURING GROWTH ON MUCIN
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DOI：
10.1099/13500872-140-12-3407
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发表时间：
1994-12-01
期刊：
影响因子：
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通讯作者：
BEIGHTON, D
中科院分区：
文献类型：
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作者：
BRADSHAW, DJ;HOMER, KA;BEIGHTON, D
猪胃粘蛋白已被用作模型糖蛋白，以确定不同口腔细菌产生的特定糖苷酶在稳定、多样化微生物群落发展中的作用。用合成底物对10种口腔细菌进行了糖苷酶和蛋白酶活性的纯培养。在恒化器中建立了一个五元混合培养，其中包括糖苷酶和蛋白酶活性最低的物种，其中猪胃粘蛋白是主要的碳和能量来源。引入具有新酶活性的其他物种（如唾液酸酶、α -聚焦酶和内多肽酶），使它们在群落中建立起7个、8个、9个和10个成员的群落，并导致微生物群落的总活菌数增加。这种活菌数量的增加是由新增加的物种数量和现有群落成员数量的增加组成的。这一结果表明，糖蛋白的分解代谢涉及多个物种的协同和协调作用，具有重叠的酶活性模式。这种代谢合作导致了额外营养物质的释放，这可能有助于维持在许多自然栖息地中发现的常驻微生物群落的特征多样性。
Hog gastric mucin has been used as a model glycoprotein to determine the role of particular glycosidases produced by different oral bacteria in the development of stable, diverse microbial communities. The patterns of glycosidase and protease activity were determined in pure cultures of ten representative species of oral bacteria using synthetic substrates. A five-member mixed culture was established in a chemostat, comprising species with minimal glycosidase and protease activity, in which hog gastric mucin was the major carbon and energy source. Introduction of additional species with novel enzyme activities (e.g. sialidase, alpha-fucosidase and endopeptidase) led to their establishment within the community to make communities with seven, eight, nine and ten members and resulted in an increase in the total viable counts of the microbial consortium. This increase in viable count was made up of the numbers of the newly added species as well as from a rise in the numbers of the existing community members. This result suggested that glycoprotein catabolism involved the synergistic and concerted action of several species with overlapping patterns of enzyme activity. Such metabolic cooperation results in the liberation of additional nutrients, and this may help to maintain the characteristic diversity of resident microbial communities found in many natural habitats.