Characterization of 19 microsatellite loci in the clonal monkshood Aconitum kusnezoffii (Ranunculaceae).
Characterization of 19 microsatellite loci in the clonal monkshood Aconitum kusnezoffii (Ranunculaceae).
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DOI：
10.3732/apps.1500141
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发表时间：
2016-06
影响因子：
3.6
通讯作者：
Liao WJ
中科院分区：
文献类型：
--
作者：
Ge XY;Tian H;Liao WJ
分离微卫星基因座并从kusnezoffii（ranunculaceae）中表征，以估计男性和女性的生殖成功，并评估克隆生长对性繁殖的影响。
一种基因组富集方法用于开发微卫星标记。在三个研究的Kusnezoffii烟曲霉种群中，总共有19个微卫星基因座成功放大，其中13个基因座是多态性的。为鉴定的基因座设计的大多数底漆对也放大了Barbatuma。barbatumvar中的相应的微卫星基因座。青春期和白曲霉。
确定的微卫星基因座将有助于量化A. kusnezoffii中的男性和女性健身，并评估克隆生长对性繁殖的影响。
Microsatellite loci were isolated and characterized from Aconitum kusnezoffii (Ranunculaceae) to estimate male and female reproductive success and evaluate the effects of clonal growth on sexual reproduction. A genomic enrichment approach was used to develop microsatellite markers. In three investigated A. kusnezoffii populations, a total of 19 microsatellite loci were successfully amplified, and 13 of these loci were polymorphic. Most of the primer pairs designed for the identified loci also amplified corresponding microsatellite loci in A. barbatum var. puberulum and A. alboviolaceum. The identified microsatellite loci will be useful for quantifying male and female fitness in A. kusnezoffii and evaluating the effects of clonal growth on sexual reproduction.