Regulation of the calpain and ubiquitin-proteasome systems in a canine model of muscular dystrophy.
Regulation of the calpain and ubiquitin-proteasome systems in a canine model of muscular dystrophy.
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DOI：
10.1002/mus.22125
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发表时间：
2011-10
期刊：
影响因子：
3.4
通讯作者：
Willis, Monte
中科院分区：
文献类型：
--
作者：
Wadosky, Kristine M.;Bs, Luge Li;Rodriguez, Jessica E.;Min, Jin-na;Bogan, Dan;Gonzalez, Jason;Patterson, Cam;Kornegay, Joe N.;Willis, Monte
先前的研究已经检验了钙蛋白酶和/或蛋白酶体抑制对杜氏肌营养不良症有益的假设，主要基于钙蛋白酶和蛋白酶体活性在 mdx 小鼠中增强的证据。使用 RT-PCR 测定金毛寻回犬肌营养不良模型骨骼肌和心脏中泛素蛋白酶体和钙蛋白酶系统成分的 mRNA 表达。类似地，使用荧光活性测定法测定钙蛋白酶 1/2 和蛋白酶体活性。我们发现，不到一半的测试肌肉蛋白酶体活性增加，只有一半的钙蛋白酶活性增加。此外，泛素蛋白酶体系统的转录调节在心脏中最为明显，其中许多成分显着减少。这项研究说明了泛素蛋白酶体和钙蛋白酶系统的表达和活性的多样性，这可能会导致药物抑制反应中出现意想不到的后果。
Previous studies have tested the hypothesis that calpain and/or proteasome inhibition is beneficial in Duchenne Muscular Dystrophy, based largely on evidence that calpain and proteasome activities are enhanced in the mdx mouse. mRNA expression of ubiquitin proteasome and calpain system components were determined using RT-PCR in skeletal muscle and heart in the golden retriever muscular dystrophy model. Similarly, calpain 1/2 and proteasome activities were determined using fluorometric activity assays. We found that less than half of the muscles tested had increases in proteasome activity, and only one-half had increased calpain activity. Additionally, transcriptional regulation of the ubiquitin proteasome system was most pronounced in the heart, where numerous components were significantly decreased. This study illustrates the diversity of expression and activities of the ubiquitin proteasome and calpain systems, which may lead to unexpected consequences in response to pharmacologic inhibition.
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影响因子：
5.5
作者：
Gailly, P.;De Backer, F.;Gillis, J. M.
通讯作者：
Gillis, J. M.
DOI：
10.1073/pnas.0908540107
发表时间：
2010-01-26
影响因子：
11.1
作者：
Fauconnier, Jeremy;Thireau, Jerome;Lacampagne, Alain
通讯作者：
Lacampagne, Alain
DOI：
10.1016/0006-291x(92)90242-d
发表时间：
1992-12-30
影响因子：
3.1
作者：
KAMPER, A;RODEMANN, HP
通讯作者：
RODEMANN, HP
影响因子：
12.7
作者：
Kumamoto, T;Fujimoto, S;Tsuda, T
通讯作者：
Tsuda, T
影响因子：
4.3
作者：
Bonuccelli, Gloria;Sotgia, Federica;Lisanti, Michael P.
通讯作者：
Lisanti, Michael P.