Isolation and characterization of two sorbitol transporter gene promoters in micropropagated apple plants (Malus × domestica) regulated by drought stress
Isolation and characterization of two sorbitol transporter gene promoters in micropropagated apple plants (Malus × domestica) regulated by drought stress
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DOI：
10.1007/s10725-012-9737-8
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发表时间：
2012-07
影响因子：
4.2
通讯作者：
Fang Li;H. Lei;X. Zhao;Xinjie Shen;Ailing Liu;Tianhong Li
中科院分区：
文献类型：
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作者：
Fang Li;H. Lei;X. Zhao;Xinjie Shen;Ailing Liu;Tianhong Li
许多研究已经阐明了多元醇转运蛋白在植物抗逆性中的作用。苹果中山梨醇转运蛋白基因MdSOT3、MdSOT4和MdSOT5对山梨醇的装载和卸载具有重要作用，受干旱胁迫的调控。为了进一步证实山梨醇转运蛋白在胁迫耐受性中的作用，将含有MdSOT3和MdSOT5基因的构建体引入野生型拟南芥植物(Col-0)中，并且用MdSOT3或MdSOT5转化的拟南芥与WT相比表现出更高的干旱胁迫耐受性。为了进一步了解山梨醇转运蛋白如何参与苹果植物的耐旱性，通过锚定PCR从获得的基因组DNA中分离出山梨醇转运蛋白基因的上游区域，然后用于驱动GUS报告基因在烟草植物中的表达。结果表明，MdSOT3和MdSOT5启动子的最长片段在干旱胁迫条件下诱导最高的GUS活性。此外，这些启动子的片段含有已知参与胁迫反应的顺式作用元件，也在干旱胁迫下诱导 GUS 活性。综上所述，我们的数据表明，通过这些基因启动子中的顺式作用元件增加的MdSOT3和MdSOT5活性，在赋予微繁苹果植物耐旱性方面发挥重要作用。
The role of polyol transporters in stress tolerance in plants have been elucidated by many studies. Sorbitol transporter genesMdSOT3,MdSOT4andMdSOT5in apple plants, which are important for sorbitol loading and unloading, are regulated by drought stress. To further confirm the role of sorbitol transporters in stress tolerance, the constructs harboringMdSOT3andMdSOT5genes were introduced into wild typeArabidopsisplants (Col-0) and theArabidopsistransformed withMdSOT3orMdSOT5performed higher drought stress tolerance compared to WT. In order to further understand how sorbitol transporters are involved in drought tolerance in apple plants, upstream regions of sorbitol transporter genes were isolated from apple plant source leaves by Anchored PCR from genomic DNA obtained, and then were used to drive expression of the GUS reporter in tobacco plants. The results showed that the longest fragments ofMdSOT3andMdSOT5promoters induced the highest GUS activity under drought stress conditions. Additionally, fragments of these promoters that containcis-acting elements known to be involved in stress response also induced GUS activity under drought stress. Taken together, our data suggest that increasedMdSOT3andMdSOT5activity, throughcis-acting elements in the promoters of these genes, play important roles in imparting tolerance to drought in micropropagated apple plants.