High Throughput Screening of Mitochondrial Bioenergetics in Myoblasts and Differentiated Myotubes
High Throughput Screening of Mitochondrial Bioenergetics in Myoblasts and Differentiated Myotubes
复制标题
成肌细胞和分化肌管线粒体生物能的高通量筛选
DOI：
10.1007/978-1-0716-3036-5_7
复制
发表时间：
2023
影响因子：
--
通讯作者：
Fujita Ryo
中科院分区：
文献类型：
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作者：
Takeda Kohei;Takemasa Tohru;Fujita Ryo
骨骼肌含有称为卫星细胞的干细胞，它们对于肌肉再生至关重要。卫星细胞的数量随着年龄的增长和肌肉萎缩症等病理状况的发生而下降。越来越多的证据表明，代谢开关和线粒体功能是肌发生过程中细胞命运决定（静止、激活、分化和自我更新）的关键调节因子。因此，使用Seahorse XF Bioanalyzer监测和识别活细胞中的代谢谱可以为再生和组织维护期间控制干细胞动力学的分子机制提供新的见解。在这里，我们描述了一种方法来评估线粒体呼吸（耗氧率）和糖酵解（ECAR）在原代小鼠卫星细胞，多核肌管，C2C12成肌细胞。
Skeletal muscles contain stem cells called satellite cells, which are essential for muscle regeneration. The population of satellite cells declines with aging and the incidence of pathological conditions such as muscular dystrophy. There is increasing evidence that metabolic switches and mitochondrial function are critical regulators of cell fate decision (quiescence, activation, differentiation, and self-renewal) during myogenesis. Thus, monitoring and identifying the metabolic profile in live cells using the Seahorse XF Bioanalyzer could provide new insights on the molecular mechanisms governing stem cell dynamics during regeneration and tissue maintenance. Here we described a method to assess mitochondrial respiration (oxygen consumption rate) and glycolysis (ECAR) in primary murine satellite cells, multinucleated myotubes, and C2C12 myoblasts.