Cytosolic NAD(+) content strictly depends on ATP concentration in isolated liver cells
Cytosolic NAD(+) content strictly depends on ATP concentration in isolated liver cells
复制标题
DOI：
10.1016/s0014-5793(97)00612-1
复制
发表时间：
1997-06-30
期刊：
影响因子：
3.5
通讯作者：
Rigoulet, M
中科院分区：
文献类型：
--
作者：
Devin, A;Guerin, B;Rigoulet, M
通过关注生物能转换调节中涉及的热力学关系问题，生物能研究通常考虑游离吡啶和腺嘌呤核苷酸，而不是它们在细胞质或线粒体中的总池。在这项研究中，我们报告了一个新的观察结果，在稳定状态下，生理条件下全细胞ATP含量的增加增加了烟酰胺核苷酸含量。当只考虑NAD（+）和ATP时，它是一条直线关系，当考虑这种现象的区隔时，似乎[NAD(+)]和[ATP]之间的线性关系只发生在细胞质中。这种依赖性可能是肝细胞内各种代谢途径之间调节的补充机制。(C) 1997年欧洲生化学会联合会。
By focusing on the question of the thermodynamic relationships involved in the regulation of biological energy conversion, bioenergetic studies usually consider the free pyridine and adenine nucleotide rather than their total pools, in either cytosol or mitochondria. In this study, we report a new observation that, at steady state, nicotinamide nucleotide content is increased by a rise in the ATP content of the whole cell under physiological conditions. It is a straight line relationship when only NAD(+) and ATP are considered, When regarding the compartmentation of this phenomenon, it appears that the linear relationship between [NAD(+)] and [ATP] occurs only in the cytosol. Such a dependence could be a supplementary mechanism of regulation between various metabolic pathways in the liver cell. (C) 1997 Federation of European Biochemical Societies.