INDUCTION OF PROGRAMMED CELL DEATH IN ARABIDOPSIS AND RICE BY SINGLE-WALL CARBON NANOTUBES
INDUCTION OF PROGRAMMED CELL DEATH IN ARABIDOPSIS AND RICE BY SINGLE-WALL CARBON NANOTUBES
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单壁碳纳米管诱导拟南芥和水稻程序性细胞死亡
DOI：
10.3732/ajb.1000073
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发表时间：
2010-10-01
影响因子：
3
通讯作者：
Yao, Nan
中科院分区：
文献类型：
--
作者：
Shen, Cong-Xiang;Zhang, Quan-Fang;Yao, Nan
研究前提：单壁碳纳米管（SWCNT）具有许多独特的结构和机械性能。近年来，它们的潜在应用，特别是在生物医学工程和医学化学方面，一直在增加，但纳米颗粒在植物中的毒理学影响很少被研究。方法：我们将拟南芥和水稻叶原生质体暴露于单壁碳纳米管中，并检查细胞活力、DNA损伤、活性氧生成和相关基因表达。我们还测试了注入单壁碳纳米管溶液后纳米颗粒对拟南芥叶片的影响。使用 EM-TUNEL（电子显微镜末端脱氧核苷酸转移酶介导的 dUTP 缺口末端标记）和氯化铈染色方法。关键结果：SWCNT 引起不良细胞反应，包括细胞聚集、染色质凝结以及 TUNEL 阳性反应、质膜沉积和 H2O2 积累。 SWCNT对细胞存活的影响是剂量依赖性的，25μg/mL在6小时内诱导25％的细胞死亡。相比之下，活性炭不是纳米碳颗粒，即使在处理后 24 小时也不会诱导细胞死亡。数据表明，颗粒的纳米尺寸是毒性的关键因素。此外，单壁碳纳米管处理后形成了具有氯化铈沉积物的内吞样结构，这表明纳米粒子穿过细胞膜的可能途径。结论：因此，单壁碳纳米管通过氧化应激对原生质体和叶子产生不利影响，导致一定量的程序性细胞死亡。尽管纳米材料在许多方面具有巨大优势，但其益处和副作用仍需要仔细评估。
Premise of the study : Single-walled carbon nanotubes (SWCNTs) have many unique structural and mechanical properties. Their potential applications, especially in biomedical engineering and medical chemistry, have been increasing in recent years, but the toxicological impact of nanoparticles has rarely been studied in plants.Methods : We exposed Arabidopsis and rice leaf protoplasts to SWCNTs and examined cell viability, DNA damage, reactive oxygen species generation, and related gene expression. We also tested the effects of nanoparticles on Arabidopsis leaves after injecting a SWCNT solution. EM-TUNEL (electron-microscopic terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling) and a cerium chloride staining method were used.Key results : SWCNTs caused adverse cellular responses including cell aggregation, chromatin condensation along with a TUNEL-positive reaction, plasma membrane deposition, and H2O2 accumulation. The effect of SWCNTs on the survival of cells was dose dependent, with 25 mu g/mL inducing 25% cell death in 6 h. In contrast, activated carbon, which is not a nanosized carbon particle, did not induce cell death even 24 h after treatments. The data indicated that the nano-size of the particle is a critical factor for toxicity. Moreover, endocytosis-like structures with cerium chloride deposits formed after SWCNT treatment, suggesting a possible pathway for nanoparticles to traverse the cell membrane.Conclusions : Consequently, SWCNTs have an adverse effect on protoplasts and leaves through oxidative stress, leading to a certain amount of programmed cell death. Although nanomaterials have great advantages in many respects, the benefits and side effects still need to be assessed carefully.