Characterization of Urine-Derived Stem Cells Obtained from Upper Urinary Tract for Use in Cell-Based Urological Tissue Engineering
Characterization of Urine-Derived Stem Cells Obtained from Upper Urinary Tract for Use in Cell-Based Urological Tissue Engineering
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DOI：
10.1089/ten.tea.2010.0637
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发表时间：
2011-08-01
影响因子：
4.1
通讯作者：
Zhang, Yuanyuan
中科院分区：
文献类型：
--
作者：
Bharadwaj, Shantaram;Liu, Guihua;Zhang, Yuanyuan
背景资料：本研究的目的是表征从上尿路（uUSC）获得的尿源性干细胞，诱导这些细胞分化为尿路上皮细胞和平滑肌细胞，并确定它们是否可以作为一个潜在的干细胞来源膀胱组织engineering.Materials和方法：尿液样本收集从5例正常的上尿路肾盂成形术。从该尿液中分离细胞并在vitro.Results中广泛扩增：uUSC的平均群体倍增为46.5 +/- 7.7。uUSC表达与间充质干细胞和周细胞相关的表面标志物。当暴露于TGF-β 1和PDGF-BB时，这些细胞可以分化成表达平滑肌特异性基因转录物和蛋白质的平滑肌样细胞，包括α-平滑肌肌动蛋白、结蛋白和肌球蛋白。在胶原晶格分析中，这些成肌分化的uUSC显示出与天然平滑肌细胞中所见相似的收缩功能。结论：uUSC具有体外扩增和分化的能力，可作为膀胱组织工程的替代细胞来源。
Background: The goals of this study were to characterize urine-derived stem cells obtained from the upper urinary tract (uUSC), induce these cells to differentiate into urothelial and smooth muscle cells, and determine whether they could serve as a potential stem cell source for bladder tissue engineering.Materials and Methods: Urine samples were collected from five patients with normal upper urinary tracts during renal pyeloplasty. Cells were isolated from this urine and extensively expanded in vitro.Results: The mean population doubling of uUSC was 46.5 +/- 7.7. The uUSC expressed surface markers associated with mesenchymal stem cells and pericytes. These cells could differentiate into smooth muscle-like cells that expressed smooth muscle-specific gene transcripts and proteins, including a-smooth muscle actin, desmin, and myosin, when exposed to TGF-beta 1 and PDGF-BB. In a collagen lattice assay, these myogenic-differentiated uUSC displayed contractile function that was similar to that seen in native smooth muscle cells. Urothelial-differentiated uUSC expressed urothelial-specific genes and proteins such as uroplakin-Ia and -III, cytokeratin (CK)-7, and CK-13.Conclusions: uUSC possess expansion and differentiation (urothelial and myogenic) capabilities, and can potentially be used as an alternative cell source in bladder tissue engineering for patients needing cystoplasty.