Pulsed-field gel electrophoresis for genomic analysis of Neisseria gonorrhoeae.
Pulsed-field gel electrophoresis for genomic analysis of Neisseria gonorrhoeae.
复制标题
用于淋病奈瑟菌基因组分析的脉冲场凝胶电泳。
DOI：
10.1093/infdis/171.2.455
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Roberts,MC
中科院分区：
文献类型：
--
作者：
Xia,M;Whittington,WL;Holmes,KK;Plummer,FA;Roberts,MC
脉冲场凝胶电泳（PFGE）是一种用于分析大DNA片段的技术。将PFGE与缺陷型和Porserovar（A/S）分类进行比较，以确定其在淋球菌感染研究、A/S亚型分型和克隆鉴定中的实用性。2株分离株经50次传代后，PFGE图谱稳定，性伴侣分离株的PFGE图谱与两种酶均无法区分。14个来自肯尼亚的需要脯氨酸的IA-6分离株产生8个NheI和7个XbaI模式;这些分离株包括6个携带泰特M的分离株，它们都产生相同的NheI模式。1993年从美国太平洋西北部和夏威夷分离的14个精氨酸-、次黄嘌呤-和尿嘧啶-需要的IA-1，2分离株的PFGE模式与两种酶的差异较小。PFGE分析代表了一个潜在的有用的除了目前的淋球菌分类系统。
Pulsed-field gel electrophoresis (PFGE) is a technique for analyzing large DNA fragments. PFGE was compared to auxotyping andPorserovar (A/S) classification to determine its utility in the study of gonococcal infection, subtyping A/S classes, and clone identification. PFGE patterns were stable in vitro after 2 isolates were passaged 50 times, and those from sex partners were indistinguishable with both enzymes. Fourteen proline-requiring IA-6 isolates from Kenya produced 8NheIand 7XbaIpatterns; these included 6 Tet M-carrying isolates, all producing the sameNheIpattern. PFGE patterns of 14 arginine-, hypoxanthine-, and uracil-requiring IA-l,2 isolates, from the US Pacific Northwest and Hawaii in 1993, were less diverse with both enzymes. PFGE analysis represents a potentially useful addition to the current gonococcal classification system.