The comparison between toxic Pseudo-nitzschia multiseries (Hasle) Hasle and non-toxic P. pungens (Grunow) Hasle isolated from Jinhae Bay, Korea
The comparison between toxic Pseudo-nitzschia multiseries (Hasle) Hasle and non-toxic P. pungens (Grunow) Hasle isolated from Jinhae Bay, Korea
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韩国镇海湾有毒拟菱形藻 (Hasle) Hasle 与无毒 P. pungens (Grunow) Hasle 的比较
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发表时间：
2001
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通讯作者：
E. Cho
中科院分区：
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作者：
E. Cho
通过比较多系拟菱形藻和刺菱形藻的形态、产毒、生长和ITS序列，研究了两种菱形藻的生态学特性。透射电镜观察发现，刺毛蕨10厘米有9-12个腓骨，2排孔状体，1厘米有3-4个孔状体，10厘米有9-11条纹;多列毛蕨10厘米有13-14个腓骨，3-4排孔状体，1厘米有4-6个孔状体，10厘米有13-14条纹。采用高效液相色谱-紫外检测法和荧光检测法测定了多系毕赤酵母在100 μmol·L-1光照条件下，20℃培养20 d后，其全培养液、无细胞培养液和细胞组分中软骨藻酸（DA）的含量分别为221.8 ng·L-1、196.4 ng·L-1和25.4 ng·L-1，而尖锐毕赤酵母为阴性。多系和刺序拟青霉在较高温度下生长速率均较高，其中在20℃时生长速率最高。多系拟菱形藻适宜生长的盐度范围较宽（20-40 psu），但在40 psu和50 psu时，尖菱形藻的生长速率低于多系拟菱形藻。特别是，10 psu不允许任何P. mutiseries的生长。对于硝酸盐、铵和尿素生长的培养物，营养浓度对生长的变化有贡献，其中在100 μM下获得最佳生长。然而，多系原球茎即使在不含尿素的培养基中也生长良好，而刺原球茎的生长速率显著不同（p
We studied the ecological characteristics of Pseudo-nitzschia multiseries and P. pungens, comparing the morphology, toxin production, growth and ITS sequences. On the basis of transmission electron microscopy (TEM), it was found that P. pungens had 9-12 fibulae in 10㎛, 2 rows of poroids, 3-4 poroids in 1 ㎛ and 9-11 striae in 10 ㎛, while in P. multiseries 13-14 fibulae in 10 ㎛, 3-4 rows of poroids, 4-6 poroids in 1 ㎛ and 13-14 striae in 10 ㎛. Domoic acid (DA) production by P. multiseries was determined by HPLC with UV or fluorometric detection, and resulted in 221.8 ng·, 196.4 ng· and 25.4 ng· in the whole culture, cell-free medium and cell fraction, respectively, for 20 days at 20℃ under light intensity of 100 μmol· with 16:8 LD cycle, while values for P. pungens were negative. Both P.multiseries and P. pungens showed a higher growth rate at higher temperature with the highest growth rate at 20℃. Pseudo-nitzschia multiseries had a wide range of salinity (20-40 psu) for optimal growth, but at 40 psu and 50 psu, the growth rate of P. pungens was lower than that of P. multiseries. In particular, 10 psu did not allow any growth of P. mutiseries. For nitrate, ammonium and urea-grown cultures, nutrient concentration contributed to a variation of growth, in which the best growth was obtained at 100 μM.However, P. multiseries grew well even in a medium without urea, while the growth rate of P. pungens was significantly different (p