Greater enhancement of Bacillus subtilis spore yields in submerged cultures by optimization of medium composition through statistical experimental designs
Greater enhancement of Bacillus subtilis spore yields in submerged cultures by optimization of medium composition through statistical experimental designs
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DOI：
10.1007/s00253-009-2162-x
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发表时间：
2010-02-01
影响因子：
5
通讯作者：
Chen, Xiang-Dong
中科院分区：
文献类型：
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作者：
Chen, Zhen-Min;Li, Qing;Chen, Xiang-Dong
枯草芽孢杆菌芽胞制剂是一种很有前途的益生菌和生防剂，可用于植物、动物和人类。这项工作的目的是利用统计方法优化枯草杆菌(WHK-Z12)孢子生产的营养条件。初步试验表明，玉米淀粉、玉米粉和麦皮是最好的碳源。采用Plackett-Burman设计，确定玉米浆、大豆粉和酵母膏是提高产孢量的最佳氮源成分，并用中心复合设计进行了进一步优化。优化培养基组成为玉米浆16.18g/L、豆粉17.53g/L、酵母膏8.14g/L。在摇瓶发酵条件下，产孢量可达1：52+/-0：06×10(10)孢子/毫升，在30-L发酵罐中培养40h，产孢量可达1：56+/-0：07×10(10)孢子/毫升。就我们所知，这些结果与记录的枯草杆菌菌株产生的孢子产量相比是有利的。
Bacillus subtilis spore preparations are promising probiotics and biocontrol agents, which can be used in plants, animals, and humans. The aim of this work was to optimize the nutritional conditions using a statistical approach for the production of B. subtilis (WHK-Z12) spores. Our preliminary experiments show that corn starch, corn flour, and wheat bran were the best carbon sources. Using Plackett-Burman design, corn steep liquor, soybean flour, and yeast extract were found to be the best nitrogen source ingredients for enhancing spore production and were studied for further optimization using central composite design. The key medium components in our optimization medium were 16.18 g/l of corn steep liquor, 17.53 g/l of soybean flour, and 8.14 g/l of yeast extract. The improved medium produced spores as high as 1: 52 +/- 0: 06 x 10(10) spores/ml under flask cultivation conditions, and 1: 56 +/- 0: 07 x 10(10) spores/ml could be achieved in a 30-l fermenter after 40 h of cultivation. To the best of our knowledge, these results compared favorably to the documented spore yields produced by B. subtilis strains.