Isolation, Identification and Characterization of Two Aluminum-Tolerant Fungi from Acidic Red Soil
Isolation, Identification and Characterization of Two Aluminum-Tolerant Fungi from Acidic Red Soil
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酸性红壤中两种耐铝真菌的分离、鉴定及表征
DOI：
10.1007/s12088-016-0586-4
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发表时间：
2016-09-01
影响因子：
3
通讯作者：
Wu, Jichun
中科院分区：
文献类型：
--
作者：
He, Genhe;Wang, Xiaodong;Wu, Jichun
以江西省某森林酸性红壤为研究对象，从土壤中分离到8株抗铝微生物。两个菌株（S4和S7）被发现是非常耐受铝浓度高达550 mmol L-1，并能生长在低pH值（3.20-3.11）。形态学和26 S rDNA序列分析表明，菌株S4属于正青霉属（Eupenicillium），而菌株S7属于未分类的毛孢科（Trichocomaceae）。进一步的研究表明，这两种菌株都被赋予了抵抗铝的能力;菌株S4积累了大量的铝，其生长受到限制的程度比菌株S7更大。菌丝体中吸附的铝量较低，培养基中保留的铝量大得多，除了培养液的颜色变化外，这意味着这两种菌株可能通过阻止铝进入细胞和通过分泌独特的代谢产物在细胞外螯合铝来抵抗铝。
Acidic red soil from a forest in Jiangxi Province was selected to isolate aluminum (Al)-resistant microbes, from which eight fungi were isolated. Two strains (S4 and S7) were found to be extremely tolerant to Al concentrations of up to 550 mmol L-1 and could grow at low pH levels (3.20-3.11). Morphological and 26S rDNA sequence analyses indicated that strain S4 belonged to Eupenicillium, while strain S7 was an unclassified Trichocomaceae. Further investigation showed that both strains were endowed with the ability to resist Al; strain S4 accumulated such a substantial amount of Al that its growth was limited to a larger extent than strain S7. The lower amounts of Al adsorbed in the mycelium and the much larger amounts of Al retained in the medium, in addition to the color change of the culture solution, implied that these two strains may resist Al by preventing Al from entering the cell and by chelating Al by secreting unique metabolites outside of the cell.