Fertilization in maize indeterminate gametophyte1 mutant
Fertilization in maize indeterminate gametophyte1 mutant
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玉米无限配子体1突变体的受精
DOI：
10.1007/s00709-004-0045-7
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发表时间：
2004-06-01
期刊：
影响因子：
2.9
通讯作者：
Zee, SY
中科院分区：
文献类型：
--
作者：
Guo, FL;Huang, BQ;Zee, SY
玉米突变体indeterminate gametophyte1成熟胚囊的细胞形态与野生型不同。约40%的ig1胚囊含有三个或更多的协同剂和两个或更多的卵细胞在微孔端。在两种增效剂的胚囊受精过程中，两种增效剂都经常退化并被两个花粉管穿透。75%含有3个或更多协同细胞的胚囊被2个或更多花粉管穿透，尽管大多数只有1个退化的协同细胞。精子细胞和卵子经常在同一个胚囊内发生多次融合，随后产生多个胚胎。约33%的ig1胚囊有两个或两个以上的中心细胞。最大的中心细胞通常靠近卵器，包含两个未融合的极核，而位于合点端的额外中心细胞通常只有一个核。受精只发生在雄性配子和最大的双核中心细胞之间。额外的中心细胞最终在受精后退化。
Mature embryo sacs of the maize mutant indeterminate gametophyte1 displayed different cellular patterns compared to those of the wild type. About 40% of the ig1 embryo sacs contained three or more synergids and two or more egg cells at the micropylar end. During fertilization in embryo sacs with two synergids, both of them frequently degenerated and were penetrated by two pollen tubes. 75% of the embryo sacs containing three or more synergid cells were penetrated by two or more pollen tubes, although most of them had only one degenerated synergid. Multiple fusions between the sperm cells and eggs frequently occurred in the same embryo sac, which subsequently generated multiple embryos. There were two or more central cells in about 33% of ig1 embryo sacs. The largest central cell was usually adjacent to the egg apparatus and contained two unfused polar nuclei, while those extra central cells located at the chalazal end usually had a single nucleus. Fertilization occurred only between the male gamete and the largest binucleate central cell. The extra central cells eventually degenerated after fertilization.