Development of multisample biological immunoassay system using HTS SQUID and magnetic nanoparticles
Development of multisample biological immunoassay system using HTS SQUID and magnetic nanoparticles
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DOI：
10.1109/tasc.2005.849988
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发表时间：
2005-06-01
影响因子：
1.8
通讯作者：
Enpuku, K
中科院分区：
文献类型：
--
作者：
Tsukamoto, A;Saitoh, K;Enpuku, K
我们开发了一个原型的磁免疫分析系统，使用高温超导体（HTS）超导量子干涉器件（SQUID）的磁免疫分析的性能和可用性进行调查。该系统设计用于测量多个样品和液体样品，并且可以在医疗设施的无屏蔽环境中工作。为了减少环境噪声的干扰，SQUID和样品被三层坡莫合金磁屏蔽覆盖。SQUID和磁屏蔽设置在一个铝盒中，该铝盒用作RF屏蔽。将具有5 x 10 mm拾波线圈的梯度计用作传感器，拾波线圈通过蓝宝石/Cu棒由液氮冷却。我们还开发了一个带有12个反应池的可重复使用的样品盘，并在一个测量序列中检测了12个样品。测量过程由计算机控制，计算机执行数据平均。直径为25 nm的Fe 3 O 4纳米颗粒。用作测试样品。在施加约0.1 T的磁场后，我们测量了来自Fe 3 O 4纳米颗粒的反射磁场。本系统可以检测30 pg的Fe 3 O 4纳米颗粒。这一结果是通过在无屏蔽实验室环境下平均100次试验获得的。100次试验的测量时间仅为100秒。
We developed a prototype magnetic immunoassay system using a high temperature superconductor (HTS) superconducting quantum interference device (SQUID) to investigate the performance and usability of the magnetic immunoassay. The system is designed to measure multiple samples and liquid samples, and it can work in an unshielded environment at a medical facility. To reduce the disturbance from environmental noise, the SQUID and samples are covered with three-layers of permalloy magnetic shield. The SQUID and magnetic shield are set in an aluminum box which acts as an RF shield. A gradiometer with a 5 x 10 mm pickup coil, which is cooled by liquid nitrogen through a sapphire/Cu rod, is used as a sensor. We also developed a nonmagnetic sample disk with 12 reaction cells and examined 12 samples in one measurement sequence. The measurement process is controlled by a computer, which perform data averaging. Fe3O4 nanoparticles with a 25-mn diameter. were used as test samples. After applying a magnetic field of about 0.1 T, we measured the remanent magnetic field from the Fe3O4 nanoparticles. The present system could detect 30 pg of Fe3O4 nanoparticles. This result was obtained by averaging 100 trials under an unshielded laboratory environment. The measurement time for 100 trials was only 100 s.