Digital Light Processing 3D Bioprinting of Gelatin-Norbornene Hydrogel for Enhanced Vascularization.
Digital Light Processing 3D Bioprinting of Gelatin-Norbornene Hydrogel for Enhanced Vascularization.
复制标题
用于增强血管化的明胶-降冰片烯水凝胶的数字光处理 3D 生物打印。
DOI：
10.1002/mabi.202300213
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发表时间：
2023
影响因子：
4.6
通讯作者：
Lin,Chien-Chi
中科院分区：
文献类型：
--
作者：
Duong,VanThuy;Lin,Chien-Chi
数字光处理（DLP）生物打印可用于制造具有复杂内部结构的体积支架，例如可灌注的血管通道。在组织制造中成功实施DLP生物打印需要使用合适的光反应生物墨水。基于降冰片烯的生物墨水已成为3D生物打印中（甲基）丙烯酸酯化大分子单体的有吸引力的替代品，这是由于其温和而快速的反应动力学，原位细胞包封的高细胞相容性以及打印后修饰或生物活性基序缀合的适应性。在这篇文章中，明胶-石墨烯（GelNB）的开发被报道为DLP 3D生物打印的光交叉生物墨水。低浓度的GelNB（2-5重量%）和聚（乙二醇）-四硫醇（PEG 4SH）是DLP打印的，具有广泛的刚度范围（G'= 120至4000 Pa）和可灌注通道。DLP打印的GelNB水凝胶具有高度的细胞相容性，正如包封的人脐静脉内皮细胞（HUVEC）的高活力所证明的那样。被包裹的HUVECs形成具有管腔结构的相互连接的微血管网络。值得注意的是，GelNB生物墨水允许QK肽（血管内皮生长因子（VEGF）模拟肽）的原位拴系和二次缀合。QK肽的掺入显著改善了DLP打印的GelNB水凝胶的内皮化和血管生成，增强了这种生物墨水系统在各种生物织物应用中的适用性。
Digital light processing (DLP) bioprinting can be used to fabricate volumetric scaffolds with intricate internal structures, such as perfusable vascular channels. The successful implementation of DLP bioprinting in tissue fabrication requires using suitable photo‐reactive bioinks. Norbornene‐based bioinks have emerged as an attractive alternative to (meth)acrylated macromers in 3D bioprinting owing to their mild and rapid reaction kinetics, high cytocompatibility for in situ cell encapsulation, and adaptability for post‐printing modification or conjugation of bioactive motifs. In this contribution, the development of gelatin‐norbornene (GelNB) is reported as a photo‐cross‐linkable bioink for DLP 3D bioprinting. Low concentrations of GelNB (2–5 wt.%) and poly(ethylene glycol)‐tetra‐thiol (PEG4SH) are DLP‐printed with a wide range of stiffness (G' ≈120 to 4000 Pa) and with perfusable channels. DLP‐printed GelNB hydrogels are highly cytocompatible, as demonstrated by the high viability of the encapsulated human umbilical vein endothelial cells (HUVECs). The encapsulated HUVECs formed an interconnected microvascular network with lumen structures. Notably, the GelNB bioink permitted both in situ tethering and secondary conjugation of QK peptide, a vascular endothelial growth factor (VEGF)‐mimetic peptide. Incorporation of QK peptide significantly improved endothelialization and vasculogenesis of the DLP‐printed GelNB hydrogels, reinforcing the applicability of this bioink system in diverse biofabrication applications.