MUTATION OF THE NON-MENDELIAN SUPPRESSOR, PSI+, IN YEAST BY HYPERTONIC MEDIA
MUTATION OF THE NON-MENDELIAN SUPPRESSOR, PSI+, IN YEAST BY HYPERTONIC MEDIA
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DOI：
10.1073/pnas.76.4.1952
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
SHERMAN, F
中科院分区：
文献类型：
--
作者：
SINGH, A;HELMS, C;SHERMAN, F
酿酒酵母中的.psi.+染色体外决定簇抑制某些UAA标记并增加UAA标记的抑制效率并增加UAA抑制子和某些移码抑制子的抑制效率。尽管.psi.+决定簇的确切性质尚不清楚，但据信它是影响翻译机制某些组成部分的自我复制细胞质因子。在本报告中，描述了有效突变或消除.psi.+决定簇的生长条件。在高渗营养培养基中培养.psi.+培养物导致在生长周期期间快速转化为主要含有.psi.-细胞的培养物。 .psi.+至.psi.-转化的动力学证实.psi.-细胞的出现是由于诱导而非对预先存在的.psi.-细胞的选择。结果表明.psi.+决定簇的复制对高渗条件敏感。
The .psi.+ extrachromosamal determinant in the yeast Saccharomyces cerevisiae suppresses certain UAA markers and increases the efficiency of suppression of UAA markers and increases the efficiency of suppression of UAA suppressors and certain frameshift suppressors. Although the exact nature of .psi.+ determinant is unknown, it is believed to be a self-replicating cytoplasmic factor affecting some component of the translational machinery. In this report growth conditions are described for efficient mutation or elimination of the .psi.+ determinant. Incubation of .psi.+ cultures in hypertonic nutrient medium resulted in rapid conversion to a culture containing predominantly .psi.- cells during the growth cycle. The kinetics of .psi.+ to .psi.- conversion established that the occurrence of .psi.- cells was due to induction and not to selection of pre-existing .psi.- cells. The results suggest that the replication of the .psi.+ determinant is sensitive to hypertonic conditions.