The restricted expression pattern of the POU factor Oct-6 during early development of the mouse nervous system
The restricted expression pattern of the POU factor Oct-6 during early development of the mouse nervous system
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DOI：
10.1016/0925-4773(95)00472-6
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发表时间：
1996-02
影响因子：
2.6
通讯作者：
R. Zwart;L. Broos;G. Grosveld;D. Meijer
中科院分区：
文献类型：
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作者：
R. Zwart;L. Broos;G. Grosveld;D. Meijer
Oct-6是一种POU转录因子，被认为在神经外胚层来源的细胞分化中起作用。为了研究Oct-6蛋白是否可以在体内神经外胚层的建立中发挥作用，我们研究了Oct-6蛋白在小鼠发育早期的表达。表达首先在卵圆筒期胚胎的原始外胚层中观察到。在原肠胚中，Oct-6蛋白存在于绒毛膜的胚外外外胚层和胚胎的前外胚层中。随着发育的进行，Oct-6表达变得更加局限于胚胎的前内侧部分，直到Oct-6阳性细胞仅在头折叠期胚胎的神经沟中观察到。在头折叠晚期胚胎中，在整个大脑的神经上皮中检测到Oct-6表达，并且随后限制于更腹侧和前部的位置。随着前神经孔的关闭，Oct-6蛋白在中脑和前脑中以节段样模式被检测到。因此，Oct-6基因的表达模式与Oct-6蛋白在体内神经外胚层的建立和区域规范中的作用一致。Oct-6基因的两波广泛诱导，一波在原始外胚层，另一波在原始大脑，随后在表达模式中进行性限制，表明该基因的调节机制。
Oct-6 is a POU transcription factor that is thought to play a role in the differentiation of cells of neuroectodermal origin. To investigate whether the Oct-6 protein could play a role in the establishment of neuroectoderm in vivo we studied the expression of the Oct-6 protein during early mouse development. Expression is first observed in the primitive ectoderm of the egg cylinder stage embryo. In gastrulating embryos, Oct-6 protein is found in the extra-embryonic ectoderm of the chorion and the anterior ectoderm of the embryo proper. As development proceeds, Oct-6 expression becomes more restricted to the anterior medial part of the embryo until Oct-6 positive cells are observed only in the neural groove of the headfold stage embryo. In the late headfold stage embryo, Oct-6 expression is detected in the neuroepithelium of the entire brain and later is restricted to a more ventral and anterior position. As the anterior neuropore closes, Oct-6 protein is detected in a segment-like pattern in the mid- and forebrain. Thus, the expression pattern of the Oct-6 gene agrees with a role for the Oct-6 protein in the establishment and regional specification of the neuroectoderm in vivo. The two waves of widespread induction of the Oct-6 gene, one in the primitive ectoderm and another in the primitive brain, both followed by a progressive restriction in the expression patterns suggest a mechanism for the regulation of the gene.