The molecular structure and multifunctionality of the cryptic plant polymer suberin
The molecular structure and multifunctionality of the cryptic plant polymer suberin
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DOI：
10.1016/j.mtbio.2019.100039
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发表时间：
2020-01-01
影响因子：
8.2
通讯作者：
Silva Pereira, C.
中科院分区：
文献类型：
--
作者：
Correia, V. G.;Bento, A.;Silva Pereira, C.
Suberin是一种植物聚酯，由多官能长链脂肪酸和甘油组成，是一种有趣的新型抗菌材料。我们纯化木栓素软木塞使用离子液体催化过程中，确保木栓素的聚合性质保持完整或仅部分裂解的甘油债券，产生最接近的原生配置reported.The化学的木栓素，无论是在原位（在低温地面软木塞）和异位（离子液体提取），阐明使用高分辨率的一维和二维的溶液状态NMR分析。采用离心分离法分离不同密度的木栓质颗粒，并对它们的单体组成、组装和杀菌效果进行了评估。木栓质分子结构的分析显示了线性脂肪族与酰基甘油酯的相对丰度，包括所有酰基甘油构型和总羰基（C=O）、游离酸端基（COOH）、OH芳香族和OH芳香族的量。Suberin离心组分显示了一般的理化性质和单体组成，并自组装成多边形结构，显示不同程度的紧凑性时，冻干。木栓素颗粒-木栓素体-显示杀菌活性对主要的人类病原菌。指纹的复杂（植物）聚酯，如木栓素的多功能性允许识别新的聚合物组件具有显着的增值性能。
Suberin, a plant polyester, consists of polyfunctional long-chain fatty acids and glycerol and is an intriguing candidate as a novel antimicrobial material. We purified suberin from cork using ionic-liquid catalysis during which the glycerol bonds that ensure the polymeric nature of suberin remained intact or were only partially cleaved-yielding the closest to a native configuration reported to date.The chemistry of suberin, both in situ (in cryogenically ground cork) and ex situ (ionic-liquid extracted), was elucidated using high-resolution one- and two-dimensional solution-state NMR analyses. Centrifugation was used to isolate suberin particles of distinct densities and their monomeric composition, assembly, and bactericidal effect, inter alia, were assessed.Analysis of the molecular structure of suberin revealed the relative abundance of linear aliphatic vs. acylglycerol esters, comprising all acylglycerol configurations and the amounts of total carbonyls (C=O), free acid end groups (COOH), OH aliphatics, and OH aromatics. Suberin centrifuged fractions revealed generic physiochemical properties and monomeric composition and self-assemble into polygonal structures that display distinct degrees of compactness when lyophilized. Suberin particles-suberinsomes-display bactericidal activity against major human pathogenic bacteria.Fingerprinting the multifunctionality of complex (plant) polyesters such as suberin allows for the identification of novel polymer assemblies with significant value-added properties.