Measurements of the mechanical properties of contracted collagen gels populated with rat fibroblasts or cardiomyocytes.
Measurements of the mechanical properties of contracted collagen gels populated with rat fibroblasts or cardiomyocytes.
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DOI：
10.1007/s10047-003-0230-z
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发表时间：
2003-01-01
期刊：
影响因子：
--
通讯作者：
Nakamura, Takao
中科院分区：
文献类型：
--
作者：
Feng, Zhonggang;Matsumoto, Toyoaki;Nakamura, Takao
本文通过单向拉伸实验研究了大鼠成纤维细胞和心肌细胞填充的收缩胶原凝胶的力学性能。在位于100 mm直径塑料皿中的硅橡胶中切割的31 x 17 mm威尔斯孔中制备填充有不同数量的大鼠成纤维细胞或心肌细胞的大鼠I型胶原-Dulbecco改良Eagle培养基（DMEM）凝胶（每种体积为2 ml，胶原浓度为0.5 mg/ml）。将相同处理的凝胶在DMEM中漂浮孵育4天，然后进行单轴拉伸试验。成纤维细胞和心肌细胞凝胶在前3天内都发生快速收缩，但对于所使用的每种细胞数量，心肌细胞凝胶始终收缩至比成纤维细胞凝胶更小的尺寸。收缩的胶原凝胶的张力-应变曲线在低应力区域表现出指数行为，随后是线性部分，最后是最大张力点，给出了测试的凝胶的极限强度。心肌细胞凝胶比成纤维细胞凝胶具有更高的张力-应变曲线。在这两种凝胶中观察到张力松弛和循环蠕变现象，这些现象与先前在人成纤维细胞收缩的胶原凝胶中观察到的现象一致。本实验表明，I型胶原凝胶可以被大鼠成纤维细胞或心肌细胞显著收缩，从而达到一定的机械强度。在这些实验中制作的收缩胶原结构具有开发用于心肌研究的组织工程结构的潜力。
In this paper the mechanical properties of contracted collagen gels populated with rat fibroblasts or cardiomyocytes were investigated by means of uniaxial tensile testing. Rat type I collagen-Dulbecco's modified Eagle's medium (DMEM) gels (each 2 ml in volume, 0.5 mg/ml collagen concentration) populated with different numbers of rat fibroblasts or cardiomyocytes were made in 31 x 17-mm wells cut in silicone rubber located in a 100-mm diameter plastic dish. Identically treated gels were incubated for 4 days floating in DMEM and then were subjected to uniaxial tensile testing. Rapid contraction occurred within the first 3 days for both the fibroblast and cardiomyocyte gels, but the cardiomyocyte gels consistently contracted to smaller sizes than the fibroblast gels for each number of cells used. The tension-strain curve of the contracted collagen gels demonstrated exponential behavior in the low stress region, followed by a linear section, and finally a maximum tension point, giving the ultimate strength of the gel tested. The cardiomyocyte gels had higher tension-strain curves than the fibroblast gels for each number of cells used. The tension relaxation and cyclic creep phenomena were observed in both kinds of gels, and these phenomena coincide with prior observations in collagen gels contracted by human fibroblasts. This experiment shows that type I collagen gels can be significantly contracted by rat fibroblasts or cardiomyocytes so as to achieve a certain mechanical strength. The contracted collagen structures made in these experiments have potential for developing tissue-engineered structures for cardiac muscle studies.