Characterization of estrogen responsive transforming activity in human breast cancer cell lines.
Characterization of estrogen responsive transforming activity in human breast cancer cell lines.
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DOI：
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发表时间：
1986-04
期刊：
影响因子：
11.2
通讯作者：
R. Dickson;S. Bates;M. McManaway;M. Lippman
中科院分区：
文献类型：
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作者：
R. Dickson;S. Bates;M. McManaway;M. Lippman
我们的特点是生产转化生长因子（TGF）的活动，由五个人乳腺癌细胞系在文化。通过诱导软琼脂中正常大鼠肾细胞的锚定非依赖性集落形成和表皮生长因子和TGF-β受体竞争研究，检测这些细胞条件培养基中TGF-α和TGF-β活性的存在。在MCF-7（一种雌激素受体阳性细胞系，其体内肿瘤发生需要雌激素）中，17 β-雌二醇诱导TGF-α样活性增加2-5倍（柱色谱法测定表观分子量为68，000和30，000）。在另外两种雌激素应答细胞系ZR-75-B和T47 D中也观察到较低幅度的雌激素诱导（1.5-2.5倍）。TGF-β在三种细胞系中的任何一种中都没有被17 β-雌二醇诱导，并且在MCF-7和T47 D细胞系中降低了高达对照的50%。在ZR-75-B细胞系中，通过放射受体测定未检测到TGF-β。研究了两种非依赖性和高度致瘤性的细胞系。MDA-MB-231细胞系产生大量TGF-α样（Mr 30，000）和TGF-β活性。在HS 578 T细胞系中，几乎检测不到TGF-α，而产生大量TGF-β。在裸鼠中的细胞系的致瘤性和任一TGF活性的产生之间没有简单的相关性可以被证明。
We have characterized the production of transforming growth factor (TGF) activities by five human breast cancer cell lines in culture. The presence of TGF-alpha and -beta activity in medium conditioned by these cells was detected by induction of anchorage-independent colony formation of normal rat kidney cells in soft agar and by epidermal growth factor and TGF-beta receptor competition studies. In MCF-7, an estrogen-receptor positive cell line which requires estrogen for tumorigenesis in vivo, 17 beta-estradiol induced a 2-5-fold increase in a TGF-alpha-like activity (apparent molecular weight, 68,000 and 30,000 by column chromatography). An estrogen induction of lower magnitude (1.5-2.5-fold) was also seen in two other estrogen responsive cell lines, ZR-75-B and T47D. TGF-beta was not induced by 17 beta-estradiol in any of the three cell lines and was decreased by up to 50% of control in the MCF-7 and T47D cell lines. TGF-beta was not detectable by radioreceptor assay in the ZR-75-B cell line. Two hormone-independent and highly tumorigenic cell lines were studied. The MDA-MB-231 cell line produced large amounts of both TGF-alpha-like (Mr 30,000) and TGF-beta activities. In the HS578T cell line, little TGF-alpha was detectable, while large amounts of TGF-beta were produced. No simple correlations between the tumorigenicity of the cell lines in nude mice and production of either TGF activity could be demonstrated.